Results

RDF/XMLNTriplesTurtleShow queryShare ▶
SubjectPredicateObject
http://purl.uniprot.org/citations/35467063http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/35467063http://www.w3.org/2000/01/rdf-schema#comment"

Objectives

Methyltransferase-like 14 (METTL14) plays an epigenetic role in various cancer through N6-methyladenosine (m6A) modification. This study sought to analyze the mechanism of METTL14 in oral squamous cell carcinoma (OSCC) cell proliferation.

Methods

Expression levels of METTL14, lncRNA metastasis associated with lung adenocarcinoma transcript 1 (lncRNA MALAT1), microRNA (miR)-224-5p, and histone lysine demethylase 2A (KDM2A) in OSCC tissues (N = 40), and cell lines (FaDu, SCC-25, CAL-27, and SCC-15) were detected. Cell viability and colony formation capacity were assessed. m6A level, stability, and subcellular localization of lncRNA MALAT1 were determined. Nude mouse xenograft tumor assay was performed to confirm the role of METTL14 in vivo.

Results

METTL14 and lncRNA MALAT1 were upregulated, and miR-224-5p was downregulated in OSCC tissues and cells. Silencing METTL14 repressed OSCC cell viability and colony formation. Overexpression of MALAT1 and KDM2A or miR-224-5p downregulation reversed the inhibition of silencing METTL14 on OSCC cell proliferation. METTL14 induced m6A modification of MALAT1 to upregulate MALAT1. MALAT1 is comparatively bound to miR-224-5p to promote KDM2A transcription. In vivo, METTL14 promoted tumor growth via regulating MALAT1/miR-224-5p/ KDM2A.

Conclusions

Overall, our findings verified the therapeutic role of silencing METTL14 in OSCC treatment through the MALAT1/miR-224-5p/KDM2A axis."xsd:string
http://purl.uniprot.org/citations/35467063http://purl.org/dc/terms/identifier"doi:10.1111/odi.14220"xsd:string
http://purl.uniprot.org/citations/35467063http://purl.uniprot.org/core/author"Li J."xsd:string
http://purl.uniprot.org/citations/35467063http://purl.uniprot.org/core/author"Wu X."xsd:string
http://purl.uniprot.org/citations/35467063http://purl.uniprot.org/core/author"He K."xsd:string
http://purl.uniprot.org/citations/35467063http://purl.uniprot.org/core/author"Momen-Heravi F."xsd:string
http://purl.uniprot.org/citations/35467063http://purl.uniprot.org/core/date"2023"xsd:gYear
http://purl.uniprot.org/citations/35467063http://purl.uniprot.org/core/name"Oral Dis"xsd:string
http://purl.uniprot.org/citations/35467063http://purl.uniprot.org/core/pages"2012-2026"xsd:string
http://purl.uniprot.org/citations/35467063http://purl.uniprot.org/core/title"Mechanism of METTL14 and m6A modification of lncRNA MALAT1 in the proliferation of oral squamous cell carcinoma cells."xsd:string
http://purl.uniprot.org/citations/35467063http://purl.uniprot.org/core/volume"29"xsd:string
http://purl.uniprot.org/citations/35467063http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/35467063
http://purl.uniprot.org/citations/35467063http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/35467063
http://purl.uniprot.org/uniprot/#_A0A0R4J041-mappedCitation-35467063http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/35467063
http://purl.uniprot.org/uniprot/#_B3KSH0-mappedCitation-35467063http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/35467063
http://purl.uniprot.org/uniprot/#_B4DTN4-mappedCitation-35467063http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/35467063
http://purl.uniprot.org/uniprot/#_B4E2F6-mappedCitation-35467063http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/35467063
http://purl.uniprot.org/uniprot/#_B4E349-mappedCitation-35467063http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/35467063
http://purl.uniprot.org/uniprot/#_B4DJF7-mappedCitation-35467063http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/35467063
http://purl.uniprot.org/uniprot/#_Q86U44-mappedCitation-35467063http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/35467063
http://purl.uniprot.org/uniprot/#_Q3UIK4-mappedCitation-35467063http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/35467063
http://purl.uniprot.org/uniprot/#_Q3U6J4-mappedCitation-35467063http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/35467063
http://purl.uniprot.org/uniprot/#_Q9HCE5-mappedCitation-35467063http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/35467063
http://purl.uniprot.org/uniprot/#_Q8C3P7-mappedCitation-35467063http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/35467063
▶