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http://purl.uniprot.org/citations/35505420http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/35505420http://www.w3.org/2000/01/rdf-schema#comment"

Background

Primary infection of Toxoplasma gondii can cause serious abnormal pregnancy outcomes such as miscarriage and stillbirth. Inhibitory molecule B7-H4 is abundantly expressed in dendritic cells (DCs) and plays an important role in maintaining immune tolerance. However, the role of B7-H4 in decidual DCs (dDCs) in T. gondii-induced abnormal pregnancy outcomes is not clear.

Methods

We established T. gondii-infected abnormal pregnancy model in wild-type (WT) and B7-H4 knockout (B7-H4-/-) pregnant mice in vivo and cultured primary human dDCs in vitro. The abnormal pregnancy outcomes were observed and the expression of B7-H4, functional molecules (CD80, CD86, and MHC-II or HLA-DR), indoleamine 2,3-dioxygenase (IDO), cytokines (IL-10 and IL-12), and signaling molecules JAK2/STAT3 in dDCs was detected by flow cytometry and Western blot.

Results

Our results showed that T. gondii infection significantly decreased B7-H4 expression in dDCs. In addition, B7-H4-/- infected pregnant mice showed much more severe abnormal pregnancy outcomes than their counterparts. Importantly, B7-H4-/- infection further regulated the expression of molecules (CD80, CD86, and MHC-II or HLA-DR), enzyme IDO, and cytokines (IL-10 and IL-12) in dDCs. We further discovered that B7-H4-/- infection impairs the JAK2/STAT3 pathway, contributing to dDC dysfunction.

Conclusions

Taken together, the results show that reduction of B7-H4 by T. gondii infection significantly modulates the decrease in cytokine IL-10 and enzyme IDO and the increase in cytokine IL-12, contributing to dDC dysfunction. Moreover, the JAK2/STAT3 pathway is involved in the regulation of B7-H4 by T. gondii infection and in the subsequent IDO and cytokine production, which ultimately contributes to abnormal pregnancy outcomes."xsd:string
http://purl.uniprot.org/citations/35505420http://purl.org/dc/terms/identifier"doi:10.1186/s13071-022-05263-1"xsd:string
http://purl.uniprot.org/citations/35505420http://purl.uniprot.org/core/author"Jiang Y."xsd:string
http://purl.uniprot.org/citations/35505420http://purl.uniprot.org/core/author"Hu X."xsd:string
http://purl.uniprot.org/citations/35505420http://purl.uniprot.org/core/author"Li Z."xsd:string
http://purl.uniprot.org/citations/35505420http://purl.uniprot.org/core/author"Liu X."xsd:string
http://purl.uniprot.org/citations/35505420http://purl.uniprot.org/core/author"Qi H."xsd:string
http://purl.uniprot.org/citations/35505420http://purl.uniprot.org/core/author"Ren L."xsd:string
http://purl.uniprot.org/citations/35505420http://purl.uniprot.org/core/author"Sun X."xsd:string
http://purl.uniprot.org/citations/35505420http://purl.uniprot.org/core/author"Zhang H."xsd:string
http://purl.uniprot.org/citations/35505420http://purl.uniprot.org/core/author"Yang C."xsd:string
http://purl.uniprot.org/citations/35505420http://purl.uniprot.org/core/author"Xie H."xsd:string
http://purl.uniprot.org/citations/35505420http://purl.uniprot.org/core/date"2022"xsd:gYear
http://purl.uniprot.org/citations/35505420http://purl.uniprot.org/core/name"Parasit Vectors"xsd:string
http://purl.uniprot.org/citations/35505420http://purl.uniprot.org/core/pages"157"xsd:string
http://purl.uniprot.org/citations/35505420http://purl.uniprot.org/core/title"B7-H4 reduction induced by Toxoplasma gondii infection results in dysfunction of decidual dendritic cells by regulating the JAK2/STAT3 pathway."xsd:string
http://purl.uniprot.org/citations/35505420http://purl.uniprot.org/core/volume"15"xsd:string
http://purl.uniprot.org/citations/35505420http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/35505420
http://purl.uniprot.org/citations/35505420http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/35505420
http://purl.uniprot.org/uniprot/#_Q7TSP5-mappedCitation-35505420http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/35505420
http://purl.uniprot.org/uniprot/Q7TSP5http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/35505420