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http://purl.uniprot.org/citations/35524422http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/35524422http://www.w3.org/2000/01/rdf-schema#comment"

Background

Papillary thyroid carcinoma (PTC) grows slowly but has a great risk of metastasis. MicroRNAs are well known as vital tumor-related gene regulators. In PTC, the role of miR-203a-3p and the underlying mechanisms remain not completely understood.

Methods

We conducted CCK8 assay, wound healing assay, transwell experiment and flow cytometry analyses to investigate the function of miRNA-203a-3p. The interaction of miRNA-203a-3p with its gene MAP3K1 was characterized by quantitative real-time polymerase chain reaction, western blotting and luciferase assay.

Results

We found that the levels of miRNA-203a-3p were statistically decreased in PTC tissues. When mimics were delivered to TPC-1 and KTC-1 cells to upregulate miR-203a-3p, it was observed that cell proliferation, metastatic abilities and cell cycle process were prevented but cell apoptosis was enhanced. Furthermore, we proved the interaction between MAP3K1 and miR-203a-3p. Intriguingly, similar to miR-203a-3p mimics, siMAP3K1 showed a tumor-suppressive effect, and this effect could be reversed when miR-203a-3p was simultaneously inhibited. Finally, selected autophagy-linked proteins such as LC3 Beclin-1 were detected and found to be increased when miR-203a-3p was upregulated or MAP3K1 was inhibited.

Conclusion

Overall, miR-203a-3p inhibits the oncogenic characteristics of TPC-1 and KTC-1 cells via suppressing MAP3K1 and activating autophagy. Our findings might enrich the understanding and the therapeutic strategies of PTC."xsd:string
http://purl.uniprot.org/citations/35524422http://purl.org/dc/terms/identifier"doi:10.1002/jcla.24470"xsd:string
http://purl.uniprot.org/citations/35524422http://purl.uniprot.org/core/author"Dai L."xsd:string
http://purl.uniprot.org/citations/35524422http://purl.uniprot.org/core/author"Zhang W."xsd:string
http://purl.uniprot.org/citations/35524422http://purl.uniprot.org/core/author"Wu X."xsd:string
http://purl.uniprot.org/citations/35524422http://purl.uniprot.org/core/author"Zhou S."xsd:string
http://purl.uniprot.org/citations/35524422http://purl.uniprot.org/core/date"2022"xsd:gYear
http://purl.uniprot.org/citations/35524422http://purl.uniprot.org/core/name"J Clin Lab Anal"xsd:string
http://purl.uniprot.org/citations/35524422http://purl.uniprot.org/core/pages"e24470"xsd:string
http://purl.uniprot.org/citations/35524422http://purl.uniprot.org/core/title"MicroRNA-203a-3p may prevent the development of thyroid papillary carcinoma via repressing MAP3K1 and activating autophagy."xsd:string
http://purl.uniprot.org/citations/35524422http://purl.uniprot.org/core/volume"36"xsd:string
http://purl.uniprot.org/citations/35524422http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/35524422
http://purl.uniprot.org/citations/35524422http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/35524422
http://purl.uniprot.org/uniprot/#_Q13233-mappedCitation-35524422http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/35524422
http://purl.uniprot.org/uniprot/#_L8ECC8-mappedCitation-35524422http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/35524422
http://purl.uniprot.org/uniprot/L8ECC8http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/35524422
http://purl.uniprot.org/uniprot/Q13233http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/35524422