| http://purl.uniprot.org/citations/35546066 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/35546066 | http://www.w3.org/2000/01/rdf-schema#comment | "Mammalian early embryo cells have complex DNA repair mechanisms to maintain genomic integrity, and homologous recombination (HR) plays the main role in response to double-strand DNA breaks (DSBs) in these cells. Polo-like kinase 1 (PLK1) participates in the HR process and its overexpression has been shown to occur in a variety of human cancers. Nevertheless, the regulatory mechanism of PLK1 remains poorly understood, especially during the S and G2 phase. Here, we show that protein phosphatase 4 catalytic subunit (PPP4C) deletion causes severe female subfertility due to accumulation of DNA damage in oocytes and early embryos. PPP4C dephosphorylated PLK1 at the S137 site, negatively regulating its activity in the DSB response in early embryonic cells. Depletion of PPP4C induced sustained activity of PLK1 when cells exhibited DNA lesions that inhibited CHK2 and upregulated the activation of CDK1, resulting in inefficient loading of the essential HR factor RAD51. On the other hand, when inhibiting PLK1 in the S phase, DNA end resection was restricted. These results demonstrate that PPP4C orchestrates the switch between high-PLK1 and low-PLK1 periods, which couple the checkpoint to HR."xsd:string |
| http://purl.uniprot.org/citations/35546066 | http://purl.org/dc/terms/identifier | "doi:10.1242/dev.200351"xsd:string |
| http://purl.uniprot.org/citations/35546066 | http://purl.uniprot.org/core/author | "Hou Y."xsd:string |
| http://purl.uniprot.org/citations/35546066 | http://purl.uniprot.org/core/author | "Wang Z.B."xsd:string |
| http://purl.uniprot.org/citations/35546066 | http://purl.uniprot.org/core/author | "Schatten H."xsd:string |
| http://purl.uniprot.org/citations/35546066 | http://purl.uniprot.org/core/author | "Sun Q.Y."xsd:string |
| http://purl.uniprot.org/citations/35546066 | http://purl.uniprot.org/core/author | "Gao S.C."xsd:string |
| http://purl.uniprot.org/citations/35546066 | http://purl.uniprot.org/core/author | "Ouyang Y.C."xsd:string |
| http://purl.uniprot.org/citations/35546066 | http://purl.uniprot.org/core/author | "Ma X.S."xsd:string |
| http://purl.uniprot.org/citations/35546066 | http://purl.uniprot.org/core/author | "Dong M.Z."xsd:string |
| http://purl.uniprot.org/citations/35546066 | http://purl.uniprot.org/core/date | "2022"xsd:gYear |
| http://purl.uniprot.org/citations/35546066 | http://purl.uniprot.org/core/name | "Development"xsd:string |
| http://purl.uniprot.org/citations/35546066 | http://purl.uniprot.org/core/pages | "dev200351"xsd:string |
| http://purl.uniprot.org/citations/35546066 | http://purl.uniprot.org/core/title | "PPP4C facilitates homologous recombination DNA repair by dephosphorylating PLK1 during early embryo development."xsd:string |
| http://purl.uniprot.org/citations/35546066 | http://purl.uniprot.org/core/volume | "149"xsd:string |
| http://purl.uniprot.org/citations/35546066 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/35546066 |
| http://purl.uniprot.org/citations/35546066 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/35546066 |
| http://purl.uniprot.org/uniprot/#_A0A0N4SV29-mappedCitation-35546066 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/35546066 |
| http://purl.uniprot.org/uniprot/#_A0A0N4SVS0-mappedCitation-35546066 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/35546066 |
| http://purl.uniprot.org/uniprot/#_A0A0U1RPK2-mappedCitation-35546066 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/35546066 |
| http://purl.uniprot.org/uniprot/#_A0A0U1RNM9-mappedCitation-35546066 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/35546066 |
| http://purl.uniprot.org/uniprot/#_Q07832-mappedCitation-35546066 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/35546066 |
| http://purl.uniprot.org/uniprot/#_A0A0J9YVI7-mappedCitation-35546066 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/35546066 |
| http://purl.uniprot.org/uniprot/#_A0A0U1RPR5-mappedCitation-35546066 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/35546066 |