| http://purl.uniprot.org/citations/35547772 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/35547772 | http://www.w3.org/2000/01/rdf-schema#comment | "Background: Microplastics (MPs) are a new global environmental threat. Previously, we showed the biodistribution of MPs using [64Cu] polystyrene (PS) and PET in mice. Here, we aimed to identify whether PS exposure has malignant effects on the stomach and induces resistance to therapy. Methods: BALB/c nude mice were fed 1.72 × 104 particles/mL of MP. We investigated PS accumulation in the stomach using radioisotope-labeled and fluorescent-conjugated PS. Further, we evaluated whether PS exposure induced cancer stemness and multidrug resistance, and whether it affected tumor development, tumor growth, and survival rate in vivo using a 4-week PS-exposed NCI-N87 mouse model. Using RNA-Seq analysis, we analyzed whether PS exposure induced gene expression changes in gastric tissues of mice. Results: PET imaging results showed that a single dose of [64Cu]-PS remained for 24 h in the mice stomach. The 4-week daily repetitive dose of fluorescent conjugated PS was deposited in the gastric tissues of mice. When PS was exposed, a 2.9-fold increase in migration rate was observed for NCI-N87 cells. Immunocytochemistry results showed decreased E-cadherin and increased N-cadherin expression, and flow cytometry, qPCR, and western blot analysis indicated a 1.9-fold increase in N-cadherin expression after PS exposure. Further, PS-induced multidrug resistance to bortezomib, paclitaxel, gefitinib, lapatinib, and trastuzumab was observed in the NCI-N87 mouse model due to upregulated CD44 expression. RNA-seq results identified increased asialoglycoprotein receptor 2 (ASGR2) expression after PS exposure, and ASGR2 knockdown decreased cell proliferation, migration, invasion, and drug resistance. Conclusion: We demonstrated that ASGR2 enhanced cancer hallmarks on PS exposure and induced resistance to chemo- and monoclonal antibody-therapy. Our preclinical findings may provide an incentive for further epidemiological studies on the role of MP exposure and its association with gastric cancer."xsd:string |
| http://purl.uniprot.org/citations/35547772 | http://purl.org/dc/terms/identifier | "doi:10.7150/thno.73226"xsd:string |
| http://purl.uniprot.org/citations/35547772 | http://purl.uniprot.org/core/author | "Kim J.S."xsd:string |
| http://purl.uniprot.org/citations/35547772 | http://purl.uniprot.org/core/author | "Kim H."xsd:string |
| http://purl.uniprot.org/citations/35547772 | http://purl.uniprot.org/core/author | "Choi E.J."xsd:string |
| http://purl.uniprot.org/citations/35547772 | http://purl.uniprot.org/core/author | "Zaheer J."xsd:string |
| http://purl.uniprot.org/citations/35547772 | http://purl.uniprot.org/core/date | "2022"xsd:gYear |
| http://purl.uniprot.org/citations/35547772 | http://purl.uniprot.org/core/name | "Theranostics"xsd:string |
| http://purl.uniprot.org/citations/35547772 | http://purl.uniprot.org/core/pages | "3217-3236"xsd:string |
| http://purl.uniprot.org/citations/35547772 | http://purl.uniprot.org/core/title | "Enhanced ASGR2 by microplastic exposure leads to resistance to therapy in gastric cancer."xsd:string |
| http://purl.uniprot.org/citations/35547772 | http://purl.uniprot.org/core/volume | "12"xsd:string |
| http://purl.uniprot.org/citations/35547772 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/35547772 |
| http://purl.uniprot.org/citations/35547772 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/35547772 |
| http://purl.uniprot.org/uniprot/#_P24721-mappedCitation-35547772 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/35547772 |
| http://purl.uniprot.org/uniprot/P24721 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/35547772 |