| http://purl.uniprot.org/citations/35549704 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/35549704 | http://www.w3.org/2000/01/rdf-schema#comment | "BackgroundIn both Drosophila and mammals, the achaete-scute (ASC/ASCL) proneural bHLH transcription factors are expressed in the developing central and peripheral nervous systems, where they function during specification and maintenance of the neural stem cells in opposition to Notch signaling. In addition to their role in nervous system development, ASC transcription factors are oncogenic and exhibit chromatin reprogramming activity; however, the impact of ASC on chromatin dynamics during neural stem cell generation remains elusive. Here, we investigate the chromatin changes accompanying neural commitment using an integrative genetics and genomics methodology.ResultsWe found that ASC factors bind equally strongly to two distinct classes of cis-regulatory elements: open regions remodeled earlier during maternal to zygotic transition by Zelda and less accessible, Zelda-independent regions. Both classes of cis-elements exhibit enhanced chromatin accessibility during neural specification and correlate with transcriptional regulation of genes involved in a variety of biological processes necessary for neuroblast function/homeostasis. We identified an ASC-Notch regulated TF network that includes likely prime regulators of neuroblast function. Using a cohort of ASC target genes, we report that ASC null neuroblasts are defectively specified, remaining initially stalled, unable to divide, and lacking expression of many proneural targets. When mutant neuroblasts eventually start proliferating, they produce compromised progeny. Reporter lines driven by proneural-bound enhancers display ASC dependency, suggesting that the partial neuroblast identity seen in the absence of ASC genes is likely driven by other, proneural-independent, cis-elements. Neuroblast impairment and the late differentiation defects of ASC mutants are corrected by ectodermal induction of individual ASC genes but not by individual members of the TF network downstream of ASC. However, in wild-type embryos, the induction of individual members of this network induces CNS hyperplasia, suggesting that they synergize with the activating function of ASC to consolidate the chromatin dynamics that promote neural specification.ConclusionsWe demonstrate that ASC proneural transcription factors are indispensable for the timely initiation of the neural stem cell program at the chromatin level by regulating a large number of enhancers in the vicinity of neural genes. This early chromatin remodeling is crucial for both neuroblast homeostasis as well as future progeny fidelity."xsd:string |
| http://purl.uniprot.org/citations/35549704 | http://purl.org/dc/terms/identifier | "doi:10.1186/s12915-022-01300-8"xsd:string |
| http://purl.uniprot.org/citations/35549704 | http://purl.uniprot.org/core/author | "Delidakis C."xsd:string |
| http://purl.uniprot.org/citations/35549704 | http://purl.uniprot.org/core/author | "Theodorou V."xsd:string |
| http://purl.uniprot.org/citations/35549704 | http://purl.uniprot.org/core/author | "Stefanaki A."xsd:string |
| http://purl.uniprot.org/citations/35549704 | http://purl.uniprot.org/core/author | "Drakos M."xsd:string |
| http://purl.uniprot.org/citations/35549704 | http://purl.uniprot.org/core/author | "Triantafyllou D."xsd:string |
| http://purl.uniprot.org/citations/35549704 | http://purl.uniprot.org/core/date | "2022"xsd:gYear |
| http://purl.uniprot.org/citations/35549704 | http://purl.uniprot.org/core/name | "BMC Biol"xsd:string |
| http://purl.uniprot.org/citations/35549704 | http://purl.uniprot.org/core/pages | "107"xsd:string |
| http://purl.uniprot.org/citations/35549704 | http://purl.uniprot.org/core/title | "ASC proneural factors are necessary for chromatin remodeling during neuroectodermal to neuroblast fate transition to ensure the timely initiation of the neural stem cell program."xsd:string |
| http://purl.uniprot.org/citations/35549704 | http://purl.uniprot.org/core/volume | "20"xsd:string |
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