| http://purl.uniprot.org/citations/35576810 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/35576810 | http://www.w3.org/2000/01/rdf-schema#comment | "Nuclear deformation is an essential phenomenon allowing cell migration and can be observed in association with pathological conditions such as laminopathies, neurodegenerative disorders and diabetes. Abnormal nuclear morphologies are a hallmark of cancer progression and nuclear deformability is a necessary feature for metastatic progression. Nevertheless, the cellular processes and the key molecular components controlling nuclear shape are poorly understood, in part due to a limited availability of assays that allow high-throughput screening of nuclear morphology-phenotypes. In this study, we explore the application of micropillared substrates as the basis for a phenotypic screening platform aimed at identifying novel determinants of nuclear morphology. We designed PDMS substrates to maximize simplicity in image acquisition and analyses, and in a small-scale screening of inhibitors targeting chromatin-modifying enzymes, we identify histone deacetylation as cellular process involved in nuclear deformation. With increasingly specific targeting approaches, we identify HDAC2 as a novel player in controlling nuclear morphology through gene transcription repression. This study shows the effectiveness of micropillar-based substrates to act as phenotypic drug screening platforms and opens a new avenue in the identification of genes involved in determining the nuclear shape."xsd:string |
| http://purl.uniprot.org/citations/35576810 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.biomaterials.2022.121564"xsd:string |
| http://purl.uniprot.org/citations/35576810 | http://purl.uniprot.org/core/author | "Jiang B."xsd:string |
| http://purl.uniprot.org/citations/35576810 | http://purl.uniprot.org/core/author | "Yang G."xsd:string |
| http://purl.uniprot.org/citations/35576810 | http://purl.uniprot.org/core/author | "Zhu X."xsd:string |
| http://purl.uniprot.org/citations/35576810 | http://purl.uniprot.org/core/author | "Cui M."xsd:string |
| http://purl.uniprot.org/citations/35576810 | http://purl.uniprot.org/core/author | "Qu S."xsd:string |
| http://purl.uniprot.org/citations/35576810 | http://purl.uniprot.org/core/author | "Elvassore N."xsd:string |
| http://purl.uniprot.org/citations/35576810 | http://purl.uniprot.org/core/author | "Grespan E."xsd:string |
| http://purl.uniprot.org/citations/35576810 | http://purl.uniprot.org/core/author | "Luni C."xsd:string |
| http://purl.uniprot.org/citations/35576810 | http://purl.uniprot.org/core/author | "Martewicz S."xsd:string |
| http://purl.uniprot.org/citations/35576810 | http://purl.uniprot.org/core/date | "2022"xsd:gYear |
| http://purl.uniprot.org/citations/35576810 | http://purl.uniprot.org/core/name | "Biomaterials"xsd:string |
| http://purl.uniprot.org/citations/35576810 | http://purl.uniprot.org/core/pages | "121564"xsd:string |
| http://purl.uniprot.org/citations/35576810 | http://purl.uniprot.org/core/title | "Micropillar-based phenotypic screening platform uncovers involvement of HDAC2 in nuclear deformability."xsd:string |
| http://purl.uniprot.org/citations/35576810 | http://purl.uniprot.org/core/volume | "286"xsd:string |
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