| http://purl.uniprot.org/citations/35589726 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/35589726 | http://www.w3.org/2000/01/rdf-schema#comment | "Mitochondrial complex I is a central metabolic enzyme that uses the reducing potential of NADH to reduce ubiquinone-10 (Q10) and drive four protons across the inner mitochondrial membrane, powering oxidative phosphorylation. Although many complex I structures are now available, the mechanisms of Q10 reduction and energy transduction remain controversial. Here, we reconstitute mammalian complex I into phospholipid nanodiscs with exogenous Q10. Using cryo-EM, we reveal a Q10 molecule occupying the full length of the Q-binding site in the 'active' (ready-to-go) resting state together with a matching substrate-free structure, and apply molecular dynamics simulations to propose how the charge states of key residues influence the Q10 binding pose. By comparing ligand-bound and ligand-free forms of the 'deactive' resting state (that require reactivating to catalyse), we begin to define how substrate binding restructures the deactive Q-binding site, providing insights into its physiological and mechanistic relevance."xsd:string |
| http://purl.uniprot.org/citations/35589726 | http://purl.org/dc/terms/identifier | "doi:10.1038/s41467-022-30506-1"xsd:string |
| http://purl.uniprot.org/citations/35589726 | http://purl.uniprot.org/core/author | "Hirst J."xsd:string |
| http://purl.uniprot.org/citations/35589726 | http://purl.uniprot.org/core/author | "Pereira C.S."xsd:string |
| http://purl.uniprot.org/citations/35589726 | http://purl.uniprot.org/core/author | "Biner O."xsd:string |
| http://purl.uniprot.org/citations/35589726 | http://purl.uniprot.org/core/author | "Wright J.J."xsd:string |
| http://purl.uniprot.org/citations/35589726 | http://purl.uniprot.org/core/author | "Chung I."xsd:string |
| http://purl.uniprot.org/citations/35589726 | http://purl.uniprot.org/core/author | "Bridges H.R."xsd:string |
| http://purl.uniprot.org/citations/35589726 | http://purl.uniprot.org/core/author | "Arantes G.M."xsd:string |
| http://purl.uniprot.org/citations/35589726 | http://purl.uniprot.org/core/author | "Ivanov B.S."xsd:string |
| http://purl.uniprot.org/citations/35589726 | http://purl.uniprot.org/core/date | "2022"xsd:gYear |
| http://purl.uniprot.org/citations/35589726 | http://purl.uniprot.org/core/name | "Nat Commun"xsd:string |
| http://purl.uniprot.org/citations/35589726 | http://purl.uniprot.org/core/pages | "2758"xsd:string |
| http://purl.uniprot.org/citations/35589726 | http://purl.uniprot.org/core/title | "Cryo-EM structures define ubiquinone-10 binding to mitochondrial complex I and conformational transitions accompanying Q-site occupancy."xsd:string |
| http://purl.uniprot.org/citations/35589726 | http://purl.uniprot.org/core/volume | "13"xsd:string |
| http://purl.uniprot.org/citations/35589726 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/35589726 |
| http://purl.uniprot.org/citations/35589726 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/35589726 |
| http://purl.uniprot.org/uniprot/#_P42026-mappedCitation-35589726 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/35589726 |
| http://purl.uniprot.org/uniprot/#_P42028-mappedCitation-35589726 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/35589726 |
| http://purl.uniprot.org/uniprot/#_P42029-mappedCitation-35589726 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/35589726 |
| http://purl.uniprot.org/uniprot/#_P52505-mappedCitation-35589726 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/35589726 |
| http://purl.uniprot.org/uniprot/#_O97725-mappedCitation-35589726 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/35589726 |
| http://purl.uniprot.org/uniprot/#_P23934-mappedCitation-35589726 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/35589726 |
| http://purl.uniprot.org/uniprot/#_P23935-mappedCitation-35589726 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/35589726 |