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http://purl.uniprot.org/citations/35613855http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/35613855http://www.w3.org/2000/01/rdf-schema#comment"

Rationale

The increased mortality and morbidity seen in critically injured patients appears associated with systemic inflammatory response syndrome (SIRS) and immune dysfunction, which ultimately predisposes to infection. Mitochondria released by injury could generate danger molecules, for example, ATP, which in turn would be rapidly scavenged by ectonucleotidases, expressed on regulatory immune cells.

Objective

To determine the association between circulating mitochondria, purinergic signalling and immune dysfunction after trauma.

Methods

We tested the impact of hepatocyte-derived free mitochondria on blood-derived and lung-derived CD8 T cells in vitro and in experimental mouse models in vivo. In parallel, immune phenotypic analyses were conducted on blood-derived CD8 T cells obtained from trauma patients.

Results

Isolated intact mitochondria are functional and generate ATP ex vivo. Extracellular mitochondria perturb CD8+ T cells in co-culture, inducing select features of immune exhaustion in vitro. These effects are modulated by scavenging ATP, modelled by addition of apyrase in vitro. Injection of intact mitochondria into recipient mice markedly upregulates the ectonucleotidase CD39, and other immune checkpoint markers in circulating CD8+ T cells. We note that mice injected with mitochondria, prior to instilling bacteria into the lung, exhibit more severe lung injury, characterised by elevated neutrophil influx and by changes in CD8+ T cell cytotoxic capacity. Importantly, the development of SIRS in injured humans, is likewise associated with disordered purinergic signalling and CD8 T cell dysfunction.

Conclusion

These studies in experimental models and in a cohort of trauma patients reveal important associations between extracellular mitochondria, aberrant purinergic signalling and immune dysfunction. These pathogenic factors with immune exhaustion are linked to SIRS and could be targeted therapeutically."xsd:string
http://purl.uniprot.org/citations/35613855http://purl.org/dc/terms/identifier"doi:10.1136/thoraxjnl-2021-218047"xsd:string
http://purl.uniprot.org/citations/35613855http://purl.uniprot.org/core/author"Chen G."xsd:string
http://purl.uniprot.org/citations/35613855http://purl.uniprot.org/core/author"Zhang Q."xsd:string
http://purl.uniprot.org/citations/35613855http://purl.uniprot.org/core/author"Robson S.C."xsd:string
http://purl.uniprot.org/citations/35613855http://purl.uniprot.org/core/author"Melton D."xsd:string
http://purl.uniprot.org/citations/35613855http://purl.uniprot.org/core/author"Lee G.R."xsd:string
http://purl.uniprot.org/citations/35613855http://purl.uniprot.org/core/author"Otterbein L.E."xsd:string
http://purl.uniprot.org/citations/35613855http://purl.uniprot.org/core/author"Ledderose C."xsd:string
http://purl.uniprot.org/citations/35613855http://purl.uniprot.org/core/author"Csizmadia E."xsd:string
http://purl.uniprot.org/citations/35613855http://purl.uniprot.org/core/author"Hauser C.J."xsd:string
http://purl.uniprot.org/citations/35613855http://purl.uniprot.org/core/author"Longhi M.S."xsd:string
http://purl.uniprot.org/citations/35613855http://purl.uniprot.org/core/author"Junger W."xsd:string
http://purl.uniprot.org/citations/35613855http://purl.uniprot.org/core/author"Harbison J."xsd:string
http://purl.uniprot.org/citations/35613855http://purl.uniprot.org/core/author"Tiwari-Heckler S."xsd:string
http://purl.uniprot.org/citations/35613855http://purl.uniprot.org/core/date"2023"xsd:gYear
http://purl.uniprot.org/citations/35613855http://purl.uniprot.org/core/name"Thorax"xsd:string
http://purl.uniprot.org/citations/35613855http://purl.uniprot.org/core/pages"151-159"xsd:string
http://purl.uniprot.org/citations/35613855http://purl.uniprot.org/core/title"Extracellular mitochondria drive CD8 T cell dysfunction in trauma by upregulating CD39."xsd:string
http://purl.uniprot.org/citations/35613855http://purl.uniprot.org/core/volume"78"xsd:string
http://purl.uniprot.org/citations/35613855http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/35613855
http://purl.uniprot.org/citations/35613855http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/35613855
http://purl.uniprot.org/uniprot/#_P49961-mappedCitation-35613855http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/35613855
http://purl.uniprot.org/uniprot/#_Q921Q6-mappedCitation-35613855http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/35613855
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