http://purl.uniprot.org/citations/35678923 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/35678923 | http://www.w3.org/2000/01/rdf-schema#comment | "BackgroundCircular RNA (circRNA) is widely shown to be associated with the development of diabetic nephropathy (DN). Our study aimed to further explore the role of circ_0000064 and provide a new mechanism for its action in DN.MethodsCell models of DN in vitro were constructed by treating human renal mesangial cells (HRMCs) with high glucose (HG). The expression of circ_0000064, microRNA-424-5p (miR-424-5p) and Wnt family member 2B (WNT2B) mRNA was detected by quantitative real-time PCR (qPCR). Cell proliferation was assessed by CCK-8 assay and EdU assay. Cell cycle was characterized by DNA content using flow cytometry. The releases of pro-inflammatory factors were checked using commercial ELISA kits. The expression of cell cycle- and fibrosis-associated proteins was detected by western blot. The interplays between miR-424-5p and circ_0000064 or WNT2B were verified by dual-luciferase reporter assay and RIP assay.ResultsCirc_0000064 and WNT2B were upregulated, while miR-424-5p was downregulated in HG-treated HRMCs. Circ_0000064 knockdown largely attenuated HG-induced proliferation, inflammatory responses and extracellular matrix (ECM) accumulation in HRMCs, and miR-424-5p deficiency reversed the role of circ_0000064 knockdown. MiR-424-5p was a target of circ_0000064, and miR-424-5p directly bound to WNT2B. MiR-424-5p restoration alleviated HG-induced proliferation, inflammatory responses and ECM accumulation in HRMCs, and WNT2B overexpression partially abolished the effects of miR-424-5p.ConclusionCirc_0000064 knockdown ameliorated HG-induced HRMC dysfunctions through miR-424-5p enrichment-mediated WNT2B inhibition, hinting that circ_0000064 contributed to DN development."xsd:string |
http://purl.uniprot.org/citations/35678923 | http://purl.org/dc/terms/identifier | "doi:10.1007/s10157-022-02241-w"xsd:string |
http://purl.uniprot.org/citations/35678923 | http://purl.uniprot.org/core/author | "Li J."xsd:string |
http://purl.uniprot.org/citations/35678923 | http://purl.uniprot.org/core/author | "Zhao Q."xsd:string |
http://purl.uniprot.org/citations/35678923 | http://purl.uniprot.org/core/author | "Min Y."xsd:string |
http://purl.uniprot.org/citations/35678923 | http://purl.uniprot.org/core/date | "2022"xsd:gYear |
http://purl.uniprot.org/citations/35678923 | http://purl.uniprot.org/core/name | "Clin Exp Nephrol"xsd:string |
http://purl.uniprot.org/citations/35678923 | http://purl.uniprot.org/core/pages | "943-954"xsd:string |
http://purl.uniprot.org/citations/35678923 | http://purl.uniprot.org/core/title | "Circ_0000064 knockdown attenuates high glucose-induced proliferation, inflammation and extracellular matrix deposition of mesangial cells through miR-424-5p-mediated WNT2B inhibition in cell models of diabetic nephropathy."xsd:string |
http://purl.uniprot.org/citations/35678923 | http://purl.uniprot.org/core/volume | "26"xsd:string |
http://purl.uniprot.org/citations/35678923 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/35678923 |
http://purl.uniprot.org/citations/35678923 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/35678923 |
http://purl.uniprot.org/uniprot/#_Q5TEH8-mappedCitation-35678923 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/35678923 |
http://purl.uniprot.org/uniprot/#_Q93097-mappedCitation-35678923 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/35678923 |
http://purl.uniprot.org/uniprot/Q93097 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/35678923 |
http://purl.uniprot.org/uniprot/Q5TEH8 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/35678923 |