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http://purl.uniprot.org/citations/35703949http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
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Objectives

Free fatty acid receptor 4 (FFAR4) is a G-protein-coupled membrane receptor highly expressed in macrophages that triggers anti-inflammatory effects and promotes insulin sensitization. We have previously found significant associations between the FFAR4 rs11187533 single nucleotide polymorphism (SNP) and various obesity comorbidity parameters. We aimed to verify the FFAR4 expression levels in children with obesity and the associated comorbidities.

Methods

Thirty-eight children with obesity were studied. Clinical and anthropometric evaluation was performed. A venous sample under fasting conditions was obtained. Biochemical study included parameters of metabolic risk. DNA was extracted and genotyped for the rs11187533 FFAR4 SNP. Real-time PCR technique was performed to investigate the gene expression. Relative FFAR4 mRNA levels were determined according to the 2-ΔΔCt method.

Results

Significant differences in FFAR4 expression levels between the CC and CT-TT genotypes of the rs11187533 FFAR4 SNP were observed (P = 0.034). The minor allele T presented higher levels of FFAR4 expression. We found that a loss of FFAR4 expression was associated with extreme obesity (P = 0.032). The lowest FFAR4 expression levels were observed in children who had higher insulin (P = 0.008) and homeostasis model assessment insulin resistance values (P = 0.012) and lower quantitative insulin-sensitivity check index (P = 0.033).

Conclusions

The underexpression of FFAR4 was associated with extreme obesity and parameters indicative of obesity comorbidities in children. This under expression could be partially influenced by the presence of the C allele rs11187533 FFAR4 SNP."xsd:string
http://purl.uniprot.org/citations/35703949http://purl.org/dc/terms/identifier"doi:10.1097/mpg.0000000000003360"xsd:string
http://purl.uniprot.org/citations/35703949http://purl.uniprot.org/core/author"Codoner-Franch P."xsd:string
http://purl.uniprot.org/citations/35703949http://purl.uniprot.org/core/author"Carrasco-Luna J."xsd:string
http://purl.uniprot.org/citations/35703949http://purl.uniprot.org/core/author"Codoner-Alejos A."xsd:string
http://purl.uniprot.org/citations/35703949http://purl.uniprot.org/core/author"Carrasco-Garcia A."xsd:string
http://purl.uniprot.org/citations/35703949http://purl.uniprot.org/core/date"2022"xsd:gYear
http://purl.uniprot.org/citations/35703949http://purl.uniprot.org/core/name"J Pediatr Gastroenterol Nutr"xsd:string
http://purl.uniprot.org/citations/35703949http://purl.uniprot.org/core/pages"535-540"xsd:string
http://purl.uniprot.org/citations/35703949http://purl.uniprot.org/core/title"Reduced Free Fatty Acid Receptor 4 Gene Expression is Associated With Extreme Obesity and Insulin Resistance in Children."xsd:string
http://purl.uniprot.org/citations/35703949http://purl.uniprot.org/core/volume"74"xsd:string
http://purl.uniprot.org/citations/35703949http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/35703949
http://purl.uniprot.org/citations/35703949http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/35703949
http://purl.uniprot.org/uniprot/#_B4DWG6-mappedCitation-35703949http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/35703949
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http://purl.uniprot.org/uniprot/B4DWG6http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/35703949