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http://purl.uniprot.org/citations/35720379http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/35720379http://www.w3.org/2000/01/rdf-schema#comment"

Introduction

Increased CCL5 expression and CD8 T cells have been shown to be pivotal regulators of immunopathology in primary Sjögren's syndrome (pSS) and pSS-like disease. Increased CCL5 expression by CCR9+ CD4 T cells has previously been implicated as a contributor to immunopathology in pSS. The role of CD8 T cells and in particular CCR9+ CD8 T cells and their potential to secrete CCL5 has not previously been studied in pSS. In this study we investigated both CCR9 and CCL5 expression by CD8 T cells in pSS patients compared to healthy controls (HC).

Methods

CCR9 expression on CD8 T cells from peripheral blood was compared between patients with pSS and HC by flow cytometry. Intracellular CCL5 expression by naive, memory and effector CCR9- and CCR9+ CD8 T cells was assessed. In addition, the capacity and pace of CCL5 release upon T cell activation was determined for all subsets and compared with CD4 T cells.

Results

The frequency of circulating CCR9+ CD8 T cells in pSS patients is increased compared to HC. Antigen-experienced CD8 T cells, especially CCR9+ effector CD8 T cells, express the highest CCL5 levels, and release the highest levels of CCL5 upon activation. Memory and effector CD8 T cells of pSS patients express significantly less CCL5 and subsequently release less CCL5 upon stimulation compared to HC. CCR9+ CD8 T cells rapidly release CCL5 and significantly more than CCR9+ CD4 T cells.

Conclusion

CCR9+ CD8 T cells express more CCL5 than CCR9-CD8 T cells. CCL5 is rapidly released upon activation, resulting in reduced intracellular expression. Reduced CCL5 expression by an elevated number of antigen-experienced CCR9-expressing CD8 T cells in pSS patients points towards increased release in vivo. This suggests that CCL5 release by CCR9+ CD8 T cells contributes to immunopathology in pSS."xsd:string
http://purl.uniprot.org/citations/35720379http://purl.org/dc/terms/identifier"doi:10.3389/fimmu.2022.887972"xsd:string
http://purl.uniprot.org/citations/35720379http://purl.uniprot.org/core/author"Leavis H.L."xsd:string
http://purl.uniprot.org/citations/35720379http://purl.uniprot.org/core/author"Kruize A.A."xsd:string
http://purl.uniprot.org/citations/35720379http://purl.uniprot.org/core/author"van Roon J.A.G."xsd:string
http://purl.uniprot.org/citations/35720379http://purl.uniprot.org/core/author"Blokland S.L.M."xsd:string
http://purl.uniprot.org/citations/35720379http://purl.uniprot.org/core/author"Hinrichs A.C."xsd:string
http://purl.uniprot.org/citations/35720379http://purl.uniprot.org/core/author"Lafeber F.P.J."xsd:string
http://purl.uniprot.org/citations/35720379http://purl.uniprot.org/core/date"2022"xsd:gYear
http://purl.uniprot.org/citations/35720379http://purl.uniprot.org/core/name"Front Immunol"xsd:string
http://purl.uniprot.org/citations/35720379http://purl.uniprot.org/core/pages"887972"xsd:string
http://purl.uniprot.org/citations/35720379http://purl.uniprot.org/core/title"CCL5 Release by CCR9+ CD8 T Cells: A Potential Contributor to Immunopathology of Primary Sjogren's Syndrome."xsd:string
http://purl.uniprot.org/citations/35720379http://purl.uniprot.org/core/volume"13"xsd:string
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