| http://purl.uniprot.org/citations/35726092 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/35726092 | http://www.w3.org/2000/01/rdf-schema#comment | "A general method to generate biosensors for user-defined molecules could provide detection tools for a wide range of biological applications. Here, we describe an approach for the rapid engineering of biosensors using PYR1 (Pyrabactin Resistance 1), a plant abscisic acid (ABA) receptor with a malleable ligand-binding pocket and a requirement for ligand-induced heterodimerization, which facilitates the construction of sense-response functions. We applied this platform to evolve 21 sensors with nanomolar to micromolar sensitivities for a range of small molecules, including structurally diverse natural and synthetic cannabinoids and several organophosphates. X-ray crystallography analysis revealed the mechanistic basis for new ligand recognition by an evolved cannabinoid receptor. We demonstrate that PYR1-derived receptors are readily ported to various ligand-responsive outputs, including enzyme-linked immunosorbent assay (ELISA)-like assays, luminescence by protein-fragment complementation and transcriptional circuits, all with picomolar to nanomolar sensitivity. PYR1 provides a scaffold for rapidly evolving new biosensors for diverse sense-response applications."xsd:string |
| http://purl.uniprot.org/citations/35726092 | http://purl.org/dc/terms/identifier | "doi:10.1038/s41587-022-01364-5"xsd:string |
| http://purl.uniprot.org/citations/35726092 | http://purl.uniprot.org/core/author | "Li Z."xsd:string |
| http://purl.uniprot.org/citations/35726092 | http://purl.uniprot.org/core/author | "Park S.Y."xsd:string |
| http://purl.uniprot.org/citations/35726092 | http://purl.uniprot.org/core/author | "Nusinow D.A."xsd:string |
| http://purl.uniprot.org/citations/35726092 | http://purl.uniprot.org/core/author | "Wei S."xsd:string |
| http://purl.uniprot.org/citations/35726092 | http://purl.uniprot.org/core/author | "Zhong W."xsd:string |
| http://purl.uniprot.org/citations/35726092 | http://purl.uniprot.org/core/author | "Peterson F.C."xsd:string |
| http://purl.uniprot.org/citations/35726092 | http://purl.uniprot.org/core/author | "Volkman B.F."xsd:string |
| http://purl.uniprot.org/citations/35726092 | http://purl.uniprot.org/core/author | "Wheeldon I."xsd:string |
| http://purl.uniprot.org/citations/35726092 | http://purl.uniprot.org/core/author | "Cutler S.R."xsd:string |
| http://purl.uniprot.org/citations/35726092 | http://purl.uniprot.org/core/author | "Whitehead T.A."xsd:string |
| http://purl.uniprot.org/citations/35726092 | http://purl.uniprot.org/core/author | "Leonard A.C."xsd:string |
| http://purl.uniprot.org/citations/35726092 | http://purl.uniprot.org/core/author | "Beltran J."xsd:string |
| http://purl.uniprot.org/citations/35726092 | http://purl.uniprot.org/core/author | "Robertson N.R."xsd:string |
| http://purl.uniprot.org/citations/35726092 | http://purl.uniprot.org/core/author | "Medina-Cucurella A.V."xsd:string |
| http://purl.uniprot.org/citations/35726092 | http://purl.uniprot.org/core/author | "Steiner P.J."xsd:string |
| http://purl.uniprot.org/citations/35726092 | http://purl.uniprot.org/core/author | "Baumer Z.T."xsd:string |
| http://purl.uniprot.org/citations/35726092 | http://purl.uniprot.org/core/author | "Bedewitz M."xsd:string |
| http://purl.uniprot.org/citations/35726092 | http://purl.uniprot.org/core/author | "Hartley Z."xsd:string |
| http://purl.uniprot.org/citations/35726092 | http://purl.uniprot.org/core/author | "Hughes B.E."xsd:string |
| http://purl.uniprot.org/citations/35726092 | http://purl.uniprot.org/core/date | "2022"xsd:gYear |
| http://purl.uniprot.org/citations/35726092 | http://purl.uniprot.org/core/name | "Nat Biotechnol"xsd:string |
| http://purl.uniprot.org/citations/35726092 | http://purl.uniprot.org/core/pages | "1855-1861"xsd:string |