| http://purl.uniprot.org/citations/35781032 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/35781032 | http://www.w3.org/2000/01/rdf-schema#comment | "Transferrin-1 (Tsf1) is an extracellular insect protein with a high affinity for iron. The functions of Tsf1 are still poorly understood; however, Drosophila melanogaster Tsf1 has been shown to influence iron distribution in the fly body and to protect flies against some infections. The goal of this study was to better understand the physiological functions of Tsf1 in D. melanogaster by 1) investigating Tsf1 null phenotypes, 2) determining tissue-specific localization of Tsf1, 3) measuring the concentration of Tsf1 in hemolymph, 4) testing Tsf1 for bacteriostatic activity, and 5) evaluating the effect of metal and paraquat treatments on Tsf1 abundance. Flies lacking Tsf1 had more iron than wild-type flies in specialized midgut cells that take up iron from the diet; however, the absence of Tsf1 had no effect on the iron content of whole midguts, fat body, hemolymph, or heads. Thus, as previous studies have suggested, Tsf1 appears to have a minor role in iron transport. Tsf1 was abundant in hemolymph from larvae (0.4 μM), pupae (1.4 μM), adult females (4.4 μM) and adult males (22 μM). Apo-Tsf1 at 1 μM had bacteriostatic activity whereas holo-Tsf1 did not, suggesting that Tsf1 can inhibit microbial growth by sequestering iron in hemolymph and other extracellular environments. This hypothesis was supported by detection of secreted Tsf1 in tracheae, testes and seminal vesicles. Colocalization of Tsf1 with an endosome marker in oocytes suggested that Tsf1 may provide iron to developing eggs; however, eggs from mothers lacking Tsf1 had the same amount of iron as control eggs, and they hatched at a wild-type rate. Thus, the primary function of Tsf1 uptake by oocytes may be to defend against infection rather than to provide eggs with iron. In beetles, Tsf1 plays a role in protection against oxidative stress. In contrast, we found that flies lacking Tsf1 had a typical life span and greater resistance to paraquat-induced oxidative stress. In addition, Tsf1 abundance remained unchanged in response to ingestion of iron, cadmium or paraquat or to injection of iron. These results suggest that Tsf1 has a limited role in protection against oxidative stress in D. melanogaster."xsd:string |
| http://purl.uniprot.org/citations/35781032 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.ibmb.2022.103811"xsd:string |
| http://purl.uniprot.org/citations/35781032 | http://purl.uniprot.org/core/author | "Park Y."xsd:string |
| http://purl.uniprot.org/citations/35781032 | http://purl.uniprot.org/core/author | "Kanost M.R."xsd:string |
| http://purl.uniprot.org/citations/35781032 | http://purl.uniprot.org/core/author | "Gorman M.J."xsd:string |
| http://purl.uniprot.org/citations/35781032 | http://purl.uniprot.org/core/author | "Tabunoki H."xsd:string |
| http://purl.uniprot.org/citations/35781032 | http://purl.uniprot.org/core/author | "Weber J.J."xsd:string |
| http://purl.uniprot.org/citations/35781032 | http://purl.uniprot.org/core/author | "Brummett L.M."xsd:string |
| http://purl.uniprot.org/citations/35781032 | http://purl.uniprot.org/core/author | "Coca M.E."xsd:string |
| http://purl.uniprot.org/citations/35781032 | http://purl.uniprot.org/core/author | "Ragan E.J."xsd:string |
| http://purl.uniprot.org/citations/35781032 | http://purl.uniprot.org/core/date | "2022"xsd:gYear |
| http://purl.uniprot.org/citations/35781032 | http://purl.uniprot.org/core/name | "Insect Biochem Mol Biol"xsd:string |
| http://purl.uniprot.org/citations/35781032 | http://purl.uniprot.org/core/pages | "103811"xsd:string |
| http://purl.uniprot.org/citations/35781032 | http://purl.uniprot.org/core/title | "Phenotypic analyses, protein localization, and bacteriostatic activity of Drosophila melanogaster transferrin-1."xsd:string |
| http://purl.uniprot.org/citations/35781032 | http://purl.uniprot.org/core/volume | "147"xsd:string |
| http://purl.uniprot.org/citations/35781032 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/35781032 |
| http://purl.uniprot.org/citations/35781032 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/35781032 |
| http://purl.uniprot.org/uniprot/#_O97355-mappedCitation-35781032 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/35781032 |
| http://purl.uniprot.org/uniprot/#_Q9VWV6-mappedCitation-35781032 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/35781032 |
| http://purl.uniprot.org/uniprot/O97355 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/35781032 |
| http://purl.uniprot.org/uniprot/Q9VWV6 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/35781032 |