| http://purl.uniprot.org/citations/35842466 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/35842466 | http://www.w3.org/2000/01/rdf-schema#comment | "The SARS-CoV-2 nucleocapsid protein (N) is responsible for RNA binding. Here we report the crystal structure of the C-terminal domain (NCTD) in open and closed conformations and in complex with guanine triphosphate, GTP. The crystal structure and biochemical studies reveal a specific interaction between the guanine, a nucleotide enriched in the packaging signals regions of coronaviruses, and a highly conserved tryptophan residue (W330). In addition, EMSA assays with SARS-CoV-2 derived RNA hairpin loops from a putative viral packaging sequence showed the preference interaction of the N-CTD to RNA oligonucleotides containing G and the loss of the specificity in the mutant W330A. Here we propose that this interaction may facilitate the viral assembly process. In summary, we have identified a specific guanine-binding pocket in the N protein that may be used to design viral assembly inhibitors."xsd:string |
| http://purl.uniprot.org/citations/35842466 | http://purl.org/dc/terms/identifier | "doi:10.1038/s42003-022-03647-8"xsd:string |
| http://purl.uniprot.org/citations/35842466 | http://purl.uniprot.org/core/author | "Vilar M."xsd:string |
| http://purl.uniprot.org/citations/35842466 | http://purl.uniprot.org/core/author | "Franco M.L."xsd:string |
| http://purl.uniprot.org/citations/35842466 | http://purl.uniprot.org/core/author | "Rafael Ciges-Tomas J."xsd:string |
| http://purl.uniprot.org/citations/35842466 | http://purl.uniprot.org/core/date | "2022"xsd:gYear |
| http://purl.uniprot.org/citations/35842466 | http://purl.uniprot.org/core/name | "Commun Biol"xsd:string |
| http://purl.uniprot.org/citations/35842466 | http://purl.uniprot.org/core/pages | "711"xsd:string |
| http://purl.uniprot.org/citations/35842466 | http://purl.uniprot.org/core/title | "Identification of a guanine-specific pocket in the protein N of SARS-CoV-2."xsd:string |
| http://purl.uniprot.org/citations/35842466 | http://purl.uniprot.org/core/volume | "5"xsd:string |
| http://purl.uniprot.org/citations/35842466 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/35842466 |
| http://purl.uniprot.org/citations/35842466 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/35842466 |
| http://purl.uniprot.org/uniprot/#_P0DTC9-mappedCitation-35842466 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/35842466 |
| http://purl.uniprot.org/uniprot/P0DTC9 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/35842466 |