| http://purl.uniprot.org/citations/35870450 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/35870450 | http://www.w3.org/2000/01/rdf-schema#comment | "The mitochondrial Lon protease (LonP1) regulates mitochondrial health by removing redundant proteins from the mitochondrial matrix. We determined LonP1 in eight nucleotide-dependent conformational states by cryoelectron microscopy (cryo-EM). The flexible assembly of N-terminal domains had 3-fold symmetry, and its orientation depended on the conformational state. We show that a conserved structural motif around T803 with a high similarity to the trypsin catalytic triad is essential for proteolysis. We show that LonP1 is not regulated by redox potential, despite the presence of two conserved cysteines at disulfide-bonding distance in its unfoldase core. Our data indicate how sequential ATP hydrolysis controls substrate protein translocation in a 6-fold binding change mechanism. Substrate protein translocation, rather than ATP hydrolysis, is a rate-limiting step, suggesting that LonP1 is a Brownian ratchet with ATP hydrolysis preventing translocation reversal. 3-fold rocking motions of the flexible N-domain assembly may assist thermal unfolding of the substrate protein."xsd:string |
| http://purl.uniprot.org/citations/35870450 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.str.2022.06.006"xsd:string |
| http://purl.uniprot.org/citations/35870450 | http://purl.uniprot.org/core/author | "Maier T."xsd:string |
| http://purl.uniprot.org/citations/35870450 | http://purl.uniprot.org/core/author | "Abrahams J.P."xsd:string |
| http://purl.uniprot.org/citations/35870450 | http://purl.uniprot.org/core/author | "Mohammed I."xsd:string |
| http://purl.uniprot.org/citations/35870450 | http://purl.uniprot.org/core/author | "Schenck N."xsd:string |
| http://purl.uniprot.org/citations/35870450 | http://purl.uniprot.org/core/author | "Topitsch A."xsd:string |
| http://purl.uniprot.org/citations/35870450 | http://purl.uniprot.org/core/author | "Balasopoulos D."xsd:string |
| http://purl.uniprot.org/citations/35870450 | http://purl.uniprot.org/core/author | "Schmitz K.A."xsd:string |
| http://purl.uniprot.org/citations/35870450 | http://purl.uniprot.org/core/date | "2022"xsd:gYear |
| http://purl.uniprot.org/citations/35870450 | http://purl.uniprot.org/core/name | "Structure"xsd:string |
| http://purl.uniprot.org/citations/35870450 | http://purl.uniprot.org/core/pages | "1254-1268.e7"xsd:string |
| http://purl.uniprot.org/citations/35870450 | http://purl.uniprot.org/core/title | "Catalytic cycling of human mitochondrial Lon protease."xsd:string |
| http://purl.uniprot.org/citations/35870450 | http://purl.uniprot.org/core/volume | "30"xsd:string |
| http://purl.uniprot.org/citations/35870450 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/35870450 |
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