| http://purl.uniprot.org/citations/35922434 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/35922434 | http://www.w3.org/2000/01/rdf-schema#comment | "The COVID-19 pandemic is exacting an increasing toll worldwide, with new SARS-CoV-2 variants emerging that exhibit higher infectivity rates and that may partially evade vaccine and antibody immunity. Rapid deployment of non-invasive therapeutic avenues capable of preventing infection by all SARS-CoV-2 variants could complement current vaccination efforts and help turn the tide on the COVID-19 pandemic. Here, we describe a novel therapeutic strategy targeting the SARS-CoV-2 RNA using locked nucleic acid antisense oligonucleotides (LNA ASOs). We identify an LNA ASO binding to the 5' leader sequence of SARS-CoV-2 that disrupts a highly conserved stem-loop structure with nanomolar efficacy in preventing viral replication in human cells. Daily intranasal administration of this LNA ASO in the COVID-19 mouse model potently suppresses viral replication (>80-fold) in the lungs of infected mice. We find that the LNA ASO is efficacious in countering all SARS-CoV-2 "variants of concern" tested both in vitro and in vivo. Hence, inhaled LNA ASOs targeting SARS-CoV-2 represents a promising therapeutic approach to reduce or prevent transmission and decrease severity of COVID-19 in infected individuals. LNA ASOs are chemically stable and can be flexibly modified to target different viral RNA sequences and could be stockpiled for future coronavirus pandemics."xsd:string |
| http://purl.uniprot.org/citations/35922434 | http://purl.org/dc/terms/identifier | "doi:10.1038/s41467-022-32216-0"xsd:string |
| http://purl.uniprot.org/citations/35922434 | http://purl.uniprot.org/core/author | "Xu L."xsd:string |
| http://purl.uniprot.org/citations/35922434 | http://purl.uniprot.org/core/author | "Lee J.Y."xsd:string |
| http://purl.uniprot.org/citations/35922434 | http://purl.uniprot.org/core/author | "Kauppinen S."xsd:string |
| http://purl.uniprot.org/citations/35922434 | http://purl.uniprot.org/core/author | "Zhu C."xsd:string |
| http://purl.uniprot.org/citations/35922434 | http://purl.uniprot.org/core/author | "Harris E."xsd:string |
| http://purl.uniprot.org/citations/35922434 | http://purl.uniprot.org/core/author | "Chiu C."xsd:string |
| http://purl.uniprot.org/citations/35922434 | http://purl.uniprot.org/core/author | "Schaletzky J."xsd:string |
| http://purl.uniprot.org/citations/35922434 | http://purl.uniprot.org/core/author | "Rouskin S."xsd:string |
| http://purl.uniprot.org/citations/35922434 | http://purl.uniprot.org/core/author | "Gonzalez F."xsd:string |
| http://purl.uniprot.org/citations/35922434 | http://purl.uniprot.org/core/author | "Pinsky B.A."xsd:string |
| http://purl.uniprot.org/citations/35922434 | http://purl.uniprot.org/core/author | "Sadreyev R.I."xsd:string |
| http://purl.uniprot.org/citations/35922434 | http://purl.uniprot.org/core/author | "Fox D."xsd:string |
| http://purl.uniprot.org/citations/35922434 | http://purl.uniprot.org/core/author | "Ji F."xsd:string |
| http://purl.uniprot.org/citations/35922434 | http://purl.uniprot.org/core/author | "Stanley S."xsd:string |
| http://purl.uniprot.org/citations/35922434 | http://purl.uniprot.org/core/author | "Blish C.A."xsd:string |
| http://purl.uniprot.org/citations/35922434 | http://purl.uniprot.org/core/author | "Rustagi A."xsd:string |
| http://purl.uniprot.org/citations/35922434 | http://purl.uniprot.org/core/author | "Yamashiro L.H."xsd:string |
| http://purl.uniprot.org/citations/35922434 | http://purl.uniprot.org/core/author | "Wehri E."xsd:string |
| http://purl.uniprot.org/citations/35922434 | http://purl.uniprot.org/core/author | "Naar A.M."xsd:string |
| http://purl.uniprot.org/citations/35922434 | http://purl.uniprot.org/core/author | "Biering S.B."xsd:string |
| http://purl.uniprot.org/citations/35922434 | http://purl.uniprot.org/core/author | "Van Dis E."xsd:string |
| http://purl.uniprot.org/citations/35922434 | http://purl.uniprot.org/core/author | "Nguyenla X."xsd:string |