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http://purl.uniprot.org/citations/35953462http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/35953462http://www.w3.org/2000/01/rdf-schema#comment"

Abstract

Aim: More patients are resuscitated from cardiac arrest and cardiopulmonary resuscitation (CA/CPR) due to advances in medical care. However, the burden now lies with post-cardiac arrest cognitive impairment in CA/CPR survivors. Based on our previous study, we aimed to further confirm the correlation between the long noncoding RNA-promoting ShcA (lncRNA-PS)/Src homology and collagen A (ShcA) axis and CA/CPR-induced cognitive impairment in molecular, cellular, and tissue levels. Methods and Results: The in vivo experiments were based on a mouse model of CA/CPR, while oxygen-glucose deprivation and reoxygenation was used as a cell model in vitro. Conditional ShcA suppression in neurons of the hippocampal CA1 region was achieved by cyclization recombinase of bacteriophage P1 recognizing DNA fragment locus of x-over P1 site (Cre/LoxP recombination system). Genetic manipulation of HT22 was achieved by lentivirus targeting lncRNA-PS and ShcA. Neurological function score was remarkably decreased, and cognitive function was affected after restoration of spontaneous circulation. LncRNA-PS and ShcA overexpression after CA/CPR, mainly happened in neurons of hippocampal CA1 region, was observed by in situ hybridization and immunofluorescence. Neuronal ShcA knockdown in hippocampal CA1 region before CA/CPR attenuated cognitive impairment after CA/CPR. ShcA deficiency protected HT22 cell line against oxygen-glucose deprivation and reoxygenation by inhibiting inflammation and apoptosis. In vitro upregulation of lncRNA-PS elevated ShcA expression, which was reversed by knockdown of ShcA. Conclusions: This study revealed that lncRNA-PS/ShcA axis is critically involved in the pathogenesis of cognitive impairment after CA/CPR. By inhibiting ShcA expression in neurons of the hippocampal CA1 region could improve the survival outcomes in mice after CA/CPR."xsd:string
http://purl.uniprot.org/citations/35953462http://purl.org/dc/terms/identifier"doi:10.1097/shk.0000000000001964"xsd:string
http://purl.uniprot.org/citations/35953462http://purl.uniprot.org/core/author"Chen C."xsd:string
http://purl.uniprot.org/citations/35953462http://purl.uniprot.org/core/author"Zhang L."xsd:string
http://purl.uniprot.org/citations/35953462http://purl.uniprot.org/core/author"Yang M."xsd:string
http://purl.uniprot.org/citations/35953462http://purl.uniprot.org/core/author"Yu H."xsd:string
http://purl.uniprot.org/citations/35953462http://purl.uniprot.org/core/author"Kang K."xsd:string
http://purl.uniprot.org/citations/35953462http://purl.uniprot.org/core/author"Peng F."xsd:string
http://purl.uniprot.org/citations/35953462http://purl.uniprot.org/core/author"Zhang Y.H."xsd:string
http://purl.uniprot.org/citations/35953462http://purl.uniprot.org/core/date"2022"xsd:gYear
http://purl.uniprot.org/citations/35953462http://purl.uniprot.org/core/name"Shock"xsd:string
http://purl.uniprot.org/citations/35953462http://purl.uniprot.org/core/pages"169-178"xsd:string
http://purl.uniprot.org/citations/35953462http://purl.uniprot.org/core/title"LONG NONCODING RNA UPREGULATES ADAPTER SHCA PROTEIN EXPRESSION TO PROMOTE COGNITIVE IMPAIRMENT AFTER CARDIAC ARREST AND RESUSCITATION."xsd:string
http://purl.uniprot.org/citations/35953462http://purl.uniprot.org/core/volume"58"xsd:string
http://purl.uniprot.org/citations/35953462http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/35953462
http://purl.uniprot.org/citations/35953462http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/35953462
http://purl.uniprot.org/uniprot/#_P98083-mappedCitation-35953462http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/35953462
http://purl.uniprot.org/uniprot/P98083http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/35953462