http://purl.uniprot.org/citations/35974445 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/35974445 | http://www.w3.org/2000/01/rdf-schema#comment | "ObjectivesIdiopathic short stature (ISS), a disorder of unknown cause, accounts for approximately 80% of the clinical diagnoses of children with short stature. Exosomal circular RNA in plasma has been implicated in various disease processes. However, the role of exosome-derived circRNA in ISS has not been elucidated yet.MethodsPlasma exosomes of ISS and normal children were cocultured with human chondrocytes. Microarray analysis and RT-PCR identified the differential expression of circRNA in exosomes between ISS and normal children. Hsa_circ_0063476 was upregulated or downregulated in human chondrocytes. Subsequently, overexpression rats of hsa_circ_0063476 was constructed via adenoviral vector to further validate the role of hsa_circ_0063476 on longitudinal bone growth via in vivo experiment.ResultsThe plasma exosome of ISS children suppressed the expression of markers of chondrocyte hypertrophy and endochondral ossification. Subsequently, upregulation of hsa_circ_0063476 in ISS exosome was identified. In vitro experiments demonstrated that chondrocyte proliferation, cell cycle and endochondral ossification were suppressed, and apoptosis was increased following hsa_circ_0063476 overexpression in human chondrocytes. Conversely, silencing hsa_circ_0063476 in human chondrocytes can show opposite outcomes. Our study further revealed hsa_circ_0063476 overexpression in vitro can enhance chondrocyte apoptosis and inhibit the expression of markers of chondrocyte proliferation and endochondral ossification via miR-518c-3p/DDX6 axis. Additionally, the rats with hsa_circ_0063476 overexpression showed a short stature phenotype.ConclusionsThe authors identified a novel pathogenesis in ISS that exosome-derived hsa_circ_0063476 retards the expression of markers of endochondral ossification and impairs longitudinal bone growth via miR-518c-3p/DDX6 axis, which may provide a unique therapeutic avenue for ISS."xsd:string |
http://purl.uniprot.org/citations/35974445 | http://purl.org/dc/terms/identifier | "doi:10.1210/endocr/bqac138"xsd:string |
http://purl.uniprot.org/citations/35974445 | http://purl.uniprot.org/core/author | "Du Z."xsd:string |
http://purl.uniprot.org/citations/35974445 | http://purl.uniprot.org/core/author | "Chen Q."xsd:string |
http://purl.uniprot.org/citations/35974445 | http://purl.uniprot.org/core/author | "Jia J."xsd:string |
http://purl.uniprot.org/citations/35974445 | http://purl.uniprot.org/core/author | "Liu X."xsd:string |
http://purl.uniprot.org/citations/35974445 | http://purl.uniprot.org/core/author | "Wu Z."xsd:string |
http://purl.uniprot.org/citations/35974445 | http://purl.uniprot.org/core/author | "Yuan J."xsd:string |
http://purl.uniprot.org/citations/35974445 | http://purl.uniprot.org/core/date | "2022"xsd:gYear |
http://purl.uniprot.org/citations/35974445 | http://purl.uniprot.org/core/name | "Endocrinology"xsd:string |
http://purl.uniprot.org/citations/35974445 | http://purl.uniprot.org/core/pages | "bqac138"xsd:string |
http://purl.uniprot.org/citations/35974445 | http://purl.uniprot.org/core/title | "Circulating Exosomal circRNA_0063476 Impairs Expression of Markers of Bone Growth Via the miR-518c-3p/DDX6 Axis in ISS."xsd:string |
http://purl.uniprot.org/citations/35974445 | http://purl.uniprot.org/core/volume | "163"xsd:string |
http://purl.uniprot.org/citations/35974445 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/35974445 |
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http://purl.uniprot.org/uniprot/B2R858 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/35974445 |