| http://purl.uniprot.org/citations/36042257 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/36042257 | http://www.w3.org/2000/01/rdf-schema#comment | "Human roundabout 1 (hRobo1) is an extracellular receptor glycoprotein that plays important roles in angiogenesis, organ development, and tumor progression. Interaction between hRobo1 and heparan sulfate (HS) has been shown to be essential for its biological activity. To better understand the effect of HS binding we engineered a lanthanide-binding peptide sequence (Loop) into the Ig2 domain of hRobo1. Native mass spectrometry was used to verify that loop introduction did not inhibit HS binding or conformational changes previously suggested by gas phase ion mobility measurements. NMR experiments measuring long-range pseudocontact shifts were then performed on 13C-methyl labeled hRobo1-Ig1-2-Loop in HS-bound and unbound forms. The magnitude of most PCSs for methyl groups in the Ig1 domain increase in the bound state confirming a change in the distribution of interdomain geometries. A grid search over Ig1 orientations to optimize the fit of data to a single conformer for both forms produced two similar structures, both of which differ from existing X-ray crystal structures and structures inferred from gas-phase ion mobility measurements. The structures and degree of fit suggest that the hRobo1-Ig1-2 structure changes slightly and becomes more rigid on HS binding. This may have implications for Robo-Slit signaling."xsd:string |
| http://purl.uniprot.org/citations/36042257 | http://purl.org/dc/terms/identifier | "doi:10.1038/s41598-022-18769-6"xsd:string |
| http://purl.uniprot.org/citations/36042257 | http://purl.uniprot.org/core/author | "Huang C."xsd:string |
| http://purl.uniprot.org/citations/36042257 | http://purl.uniprot.org/core/author | "Prestegard J.H."xsd:string |
| http://purl.uniprot.org/citations/36042257 | http://purl.uniprot.org/core/author | "Moremen K.W."xsd:string |
| http://purl.uniprot.org/citations/36042257 | http://purl.uniprot.org/core/author | "Amster I.J."xsd:string |
| http://purl.uniprot.org/citations/36042257 | http://purl.uniprot.org/core/author | "Williams R.V."xsd:string |
| http://purl.uniprot.org/citations/36042257 | http://purl.uniprot.org/core/date | "2022"xsd:gYear |
| http://purl.uniprot.org/citations/36042257 | http://purl.uniprot.org/core/name | "Sci Rep"xsd:string |
| http://purl.uniprot.org/citations/36042257 | http://purl.uniprot.org/core/pages | "14769"xsd:string |
| http://purl.uniprot.org/citations/36042257 | http://purl.uniprot.org/core/title | "NMR analysis suggests the terminal domains of Robo1 remain extended but are rigidified in the presence of heparan sulfate."xsd:string |
| http://purl.uniprot.org/citations/36042257 | http://purl.uniprot.org/core/volume | "12"xsd:string |
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