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http://purl.uniprot.org/citations/36059072http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/36059072http://www.w3.org/2000/01/rdf-schema#comment"Bridging integrator 1 (BIN1) is the second most prevalent genetic risk factor identified by genome-wide association studies (GWAS) for late-onset Alzheimer's disease. BIN1 encodes an adaptor protein that regulates membrane dynamics in the context of endocytosis and neurotransmitter vesicle release. In vitro evidence suggests that BIN1 can directly bind to tau in the cytosol. In addition, BIN1's function limits extracellular tau seed uptake by endocytosis and subsequent propagation as well as influences tau release through exosomes. However, the in vivo roles of BIN1 in tau pathogenesis and tauopathy-mediated neurodegeneration remain uncharacterized. We generated conditional knockout mice with a selective loss of Bin1 expression in the forebrain excitatory neurons and oligodendrocytes in P301S human tau transgenic background (line PS19). PS19 mice develop age-dependent tau neuropathology and motor deficits and are commonly used to study Alzheimer's disease tau pathophysiology. The severity of motor deficits and neuropathology was compared between experimental and control mice that differ with respect to forebrain BIN1 expression. BIN1's involvement in tau pathology and neuroinflammation was quantified by biochemical methods and immunostaining. Transcriptome changes were profiled by RNA-sequencing analysis to gain molecular insights. The loss of forebrain BIN1 expression in PS19 mice exacerbated tau pathology in the somatosensory cortex, thalamus, spinal cord and sciatic nerve, accelerated disease progression and caused early death. Intriguingly, the loss of BIN1 also mitigated tau neuropathology in select regions, including the hippocampus, entorhinal/piriform cortex, and amygdala, thus attenuating hippocampal synapse loss, neuronal death, neuroinflammation and brain atrophy. At the molecular level, the loss of forebrain BIN1 elicited complex neuronal and non-neuronal transcriptomic changes, including altered neuroinflammatory gene expression, concomitant with an impaired microglial transition towards the disease-associated microglial phenotype. These results provide crucial new information on in vivo BIN1 function in the context of tau pathogenesis. We conclude that forebrain neuronal BIN1 expression promotes hippocampal tau pathogenesis and neuroinflammation. Our findings highlight an exciting region specificity in neuronal BIN1 regulation of tau pathogenesis and reveal cell-autonomous and non-cell-autonomous mechanisms involved in BIN1 modulation of tau neuropathology."xsd:string
http://purl.uniprot.org/citations/36059072http://purl.org/dc/terms/identifier"doi:10.1093/brain/awac318"xsd:string
http://purl.uniprot.org/citations/36059072http://purl.uniprot.org/core/author"Zhang X."xsd:string
http://purl.uniprot.org/citations/36059072http://purl.uniprot.org/core/author"Wang S."xsd:string
http://purl.uniprot.org/citations/36059072http://purl.uniprot.org/core/author"Thinakaran G."xsd:string
http://purl.uniprot.org/citations/36059072http://purl.uniprot.org/core/author"Hansen M.T."xsd:string
http://purl.uniprot.org/citations/36059072http://purl.uniprot.org/core/author"Dammer E.B."xsd:string
http://purl.uniprot.org/citations/36059072http://purl.uniprot.org/core/author"Yuksel M."xsd:string
http://purl.uniprot.org/citations/36059072http://purl.uniprot.org/core/author"Collier L."xsd:string
http://purl.uniprot.org/citations/36059072http://purl.uniprot.org/core/author"Ponnusamy M."xsd:string
http://purl.uniprot.org/citations/36059072http://purl.uniprot.org/core/author"McMillan J.D."xsd:string
http://purl.uniprot.org/citations/36059072http://purl.uniprot.org/core/author"Blazier D.M."xsd:string
http://purl.uniprot.org/citations/36059072http://purl.uniprot.org/core/date"2023"xsd:gYear
http://purl.uniprot.org/citations/36059072http://purl.uniprot.org/core/name"Brain"xsd:string
http://purl.uniprot.org/citations/36059072http://purl.uniprot.org/core/pages"1561-1579"xsd:string
http://purl.uniprot.org/citations/36059072http://purl.uniprot.org/core/title"Loss of forebrain BIN1 attenuates hippocampal pathology and neuroinflammation in a tauopathy model."xsd:string
http://purl.uniprot.org/citations/36059072http://purl.uniprot.org/core/volume"146"xsd:string
http://purl.uniprot.org/citations/36059072http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/36059072
http://purl.uniprot.org/citations/36059072http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/36059072
http://purl.uniprot.org/uniprot/#_A0A3Q4EBK4-mappedCitation-36059072http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/36059072
http://purl.uniprot.org/uniprot/#_O08539-mappedCitation-36059072http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/36059072
http://purl.uniprot.org/uniprot/#_Q6P1B9-mappedCitation-36059072http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/36059072
http://purl.uniprot.org/uniprot/#_Q8C5M9-mappedCitation-36059072http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/36059072
http://purl.uniprot.org/uniprot/Q6P1B9http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/36059072
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