| http://purl.uniprot.org/citations/36122679 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/36122679 | http://www.w3.org/2000/01/rdf-schema#comment | "AimsTransient receptor potential melastatin-7 (TRPM7) is a selective cation permeable channel which plays important roles in cellular and developmental biology such as cell proliferation, survival, differentiation and migration. This channel is also known to be necessary for transmitter release in the peripheral nervous system. In this study, immunohistochemistry for TRPM7 was conducted in the rat lumbar dorsal root ganglion (DRG).MethodsTriple immunofluorescence methods were used to demonstrate distribution of TRPM7 and its relationship to other TRP channels in the DRG. Retrograde tracing and double immunofluorescence methods were also performed to know peripheral targets of DRG neurons containing TRPM7 and TRP vanilloid 1 (TRPV1). In addition, transection of the sciatic nerve was conducted to demonstrate an effect of the nerve injury on TRPM7expression in the DRG.ResultsTRPM7-immunoreactivity was expressed by 53.9% of sensory neurons in the 4th lumbar DRG. TRPM7-immunoreactive (-IR) DRG neurons mostly had small (<600 µm²) and medium-sized (600-1200 µm²) cell bodies. By triple and double immunofluorescence methods, approximately 70% of TRPM7-IR DRG neurons contained TRPV1-immunoreactivity. Although the number of DRG neurons co-expressing TRPM7 and TRPM8 was small in the DRG, almost all of TRPM8-IR DRG neurons co-expressed TRPM7-immunoreactivity. By combination of retrograde tracing method and immunohistochemistry, TRPM7 was expressed by half of DRG neurons innervating the plantar skin (61.9%) and gastrocnemius muscle (51.2%), and 79.6% of DRG neurons innervating the periosteum. Co-expression of TRPM7 and TRPV1 among periosteum DRG neurons (75.7%) was more abundant than among cutaneous (53.2%) and muscular (40.4%) DRG neurons. DRG neurons which co-expressed these ion channels in the periosteum had smaller cell bodies compared to the skin and muscle. In addition, the sciatic nerve transection decreased the number of TRPM7-IR neurons in the DRG (approximately 60% reduction). The RT-qPCR analysis also demonstrated reduction of TRPM7 mRNA in the injured DRG.ConclusionThe present study suggests that TRPM7 is mainly located in small nociceptors in the DRG. The content of TRPM7 in DRG neurons is probably different among their peripheral targets. TRPM7 in DRG neurons may be able to respond to noxious stimulation from their peripheral tissues. The nerve injury can decrease the level of TRPM7 mRNA and protein in DRG neurons."xsd:string |
| http://purl.uniprot.org/citations/36122679 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.jchemneu.2022.102163"xsd:string |
| http://purl.uniprot.org/citations/36122679 | http://purl.uniprot.org/core/author | "Sato T."xsd:string |
| http://purl.uniprot.org/citations/36122679 | http://purl.uniprot.org/core/author | "Kondo T."xsd:string |
| http://purl.uniprot.org/citations/36122679 | http://purl.uniprot.org/core/author | "Ichikawa H."xsd:string |
| http://purl.uniprot.org/citations/36122679 | http://purl.uniprot.org/core/author | "Yajima T."xsd:string |
| http://purl.uniprot.org/citations/36122679 | http://purl.uniprot.org/core/author | "Hosokawa H."xsd:string |
| http://purl.uniprot.org/citations/36122679 | http://purl.uniprot.org/core/date | "2022"xsd:gYear |
| http://purl.uniprot.org/citations/36122679 | http://purl.uniprot.org/core/name | "J Chem Neuroanat"xsd:string |
| http://purl.uniprot.org/citations/36122679 | http://purl.uniprot.org/core/pages | "102163"xsd:string |
| http://purl.uniprot.org/citations/36122679 | http://purl.uniprot.org/core/title | "Transient receptor potential melastatin-7 in the rat dorsal root ganglion."xsd:string |
| http://purl.uniprot.org/citations/36122679 | http://purl.uniprot.org/core/volume | "125"xsd:string |
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