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http://purl.uniprot.org/citations/36194437http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/36194437http://www.w3.org/2000/01/rdf-schema#comment"

In brief

Proper regulation of heterochromatin is critical for spermatogenesis. This study reveals the dynamic localization patterns of distinct chromatin regulators during spermatogenesis and disrupted sex chromatin status in spermatocytes in the absence of DICER.

Abstract

Heterochromatin is dynamically formed and organized in differentiating male germ cells, and its proper regulation is a prerequisite for normal spermatogenesis. While heterochromatin is generally transcriptionally silent, we have previously shown that major satellite repeat (MSR) DNA in the pericentric heterochromatin (PCH) is transcribed during spermatogenesis. We have also shown that DICER associates with PCH and is involved in the regulation of MSR-derived transcripts. To shed light on the heterochromatin regulation in the male germline, we studied the expression, localization and heterochromatin association of selected testis-enriched chromatin regulators in the mouse testis. Our results show that HELLS, WDHD1 and BAZ1A are dynamically expressed during spermatogenesis. They display limited overlap in expression, suggesting involvement in distinct heterochromatin-associated processes at different steps of differentiation. We also show that HELLS and BAZ1A interact with DICER and MSR chromatin. Interestingly, deletion of Dicer1 affects the sex chromosome heterochromatin status in late pachytene spermatocytes, as demonstrated by mislocalization of Polycomb protein family member SCML1 to the sex body. These data substantiate the importance of dynamic heterochromatin regulation during spermatogenesis and emphasize the key role of DICER in the maintenance of chromatin status in meiotic male germ cells."xsd:string
http://purl.uniprot.org/citations/36194437http://purl.org/dc/terms/identifier"doi:10.1530/rep-22-0240"xsd:string
http://purl.uniprot.org/citations/36194437http://purl.uniprot.org/core/author"Ma L."xsd:string
http://purl.uniprot.org/citations/36194437http://purl.uniprot.org/core/author"Kotaja N."xsd:string
http://purl.uniprot.org/citations/36194437http://purl.uniprot.org/core/author"Leskinen S."xsd:string
http://purl.uniprot.org/citations/36194437http://purl.uniprot.org/core/author"Makela J.A."xsd:string
http://purl.uniprot.org/citations/36194437http://purl.uniprot.org/core/author"Prakash Yadav R."xsd:string
http://purl.uniprot.org/citations/36194437http://purl.uniprot.org/core/date"2023"xsd:gYear
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http://purl.uniprot.org/citations/36194437http://purl.uniprot.org/core/pages"49-63"xsd:string
http://purl.uniprot.org/citations/36194437http://purl.uniprot.org/core/title"Chromatin remodelers HELLS, WDHD1 and BAZ1A are dynamically expressed during mouse spermatogenesis."xsd:string
http://purl.uniprot.org/citations/36194437http://purl.uniprot.org/core/volume"165"xsd:string
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