| http://purl.uniprot.org/citations/36341546 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/36341546 | http://www.w3.org/2000/01/rdf-schema#comment | "Transposable elements are a genetic reservoir from which new genes and regulatory elements can emerge. However, expression of transposable elements can be pathogenic and is therefore tightly controlled. KRAB domain-containing zinc finger proteins (KRAB-ZFPs) recruit the co-repressor KRAB-associated protein 1 (KAP1/TRIM28) to regulate many transposable elements, but how KRAB-ZFPs and KAP1 interact remains unclear. Here, we report the crystal structure of the KAP1 tripartite motif (TRIM) in complex with the KRAB domain from a human KRAB-ZFP, ZNF93. Structure-guided mutations in the KAP1-KRAB binding interface abolished repressive activity in an epigenetic transcriptional silencing assay. Deposition of H3K9me3 over thousands of loci is lost genome-wide in cells expressing a KAP1 variant with mutations that abolish KRAB binding. Our work identifies and functionally validates the KRAB-KAP1 molecular interface, which is critical for a central transcriptional control axis in vertebrates. In addition, the structure-based prediction of KAP1 recruitment efficiency will enable optimization of KRABs used in CRISPRi."xsd:string |
| http://purl.uniprot.org/citations/36341546 | http://purl.org/dc/terms/identifier | "doi:10.15252/embj.2022111179"xsd:string |
| http://purl.uniprot.org/citations/36341546 | http://purl.uniprot.org/core/author | "Modis Y."xsd:string |
| http://purl.uniprot.org/citations/36341546 | http://purl.uniprot.org/core/author | "Douse C.H."xsd:string |
| http://purl.uniprot.org/citations/36341546 | http://purl.uniprot.org/core/author | "Stoll G.A."xsd:string |
| http://purl.uniprot.org/citations/36341546 | http://purl.uniprot.org/core/author | "Pandiloski N."xsd:string |
| http://purl.uniprot.org/citations/36341546 | http://purl.uniprot.org/core/date | "2022"xsd:gYear |
| http://purl.uniprot.org/citations/36341546 | http://purl.uniprot.org/core/name | "EMBO J"xsd:string |
| http://purl.uniprot.org/citations/36341546 | http://purl.uniprot.org/core/pages | "e111179"xsd:string |
| http://purl.uniprot.org/citations/36341546 | http://purl.uniprot.org/core/title | "Structure and functional mapping of the KRAB-KAP1 repressor complex."xsd:string |
| http://purl.uniprot.org/citations/36341546 | http://purl.uniprot.org/core/volume | "41"xsd:string |
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| http://purl.uniprot.org/uniprot/#_P00720-mappedCitation-36341546 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/36341546 |
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| http://purl.uniprot.org/uniprot/B2R8R5 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/36341546 |