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http://purl.uniprot.org/citations/36344519http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/36344519http://www.w3.org/2000/01/rdf-schema#comment"A major hurdle for single particle cryo-EM in structural determination lies in the specimen preparation impaired by the air-water interface (AWI) and preferential particle-orientation problems. In this work, we develop functionalized graphene grids with various charges via a dediazoniation reaction for cryo-EM specimen preparation. The graphene grids are paraffin-assistant fabricated, which appear with less contaminations compared with those produced by polymer transfer method. By applying onto three different types of macromolecules, we demonstrate that the high-yield charged graphene grids bring macromolecules away from the AWI and enable adjustable particle-orientation distribution for more robust single particle cryo-EM structural determination."xsd:string
http://purl.uniprot.org/citations/36344519http://purl.org/dc/terms/identifier"doi:10.1038/s41467-022-34579-w"xsd:string
http://purl.uniprot.org/citations/36344519http://purl.uniprot.org/core/author"Liu Y."xsd:string
http://purl.uniprot.org/citations/36344519http://purl.uniprot.org/core/author"Lu Y."xsd:string
http://purl.uniprot.org/citations/36344519http://purl.uniprot.org/core/author"Liu N."xsd:string
http://purl.uniprot.org/citations/36344519http://purl.uniprot.org/core/author"Jia X."xsd:string
http://purl.uniprot.org/citations/36344519http://purl.uniprot.org/core/author"Yang J."xsd:string
http://purl.uniprot.org/citations/36344519http://purl.uniprot.org/core/author"Wang J."xsd:string
http://purl.uniprot.org/citations/36344519http://purl.uniprot.org/core/author"Wang H.W."xsd:string
http://purl.uniprot.org/citations/36344519http://purl.uniprot.org/core/author"Zheng L."xsd:string
http://purl.uniprot.org/citations/36344519http://purl.uniprot.org/core/author"Peng H."xsd:string
http://purl.uniprot.org/citations/36344519http://purl.uniprot.org/core/author"Rao Y."xsd:string
http://purl.uniprot.org/citations/36344519http://purl.uniprot.org/core/author"Zi Q."xsd:string
http://purl.uniprot.org/citations/36344519http://purl.uniprot.org/core/date"2022"xsd:gYear
http://purl.uniprot.org/citations/36344519http://purl.uniprot.org/core/name"Nat Commun"xsd:string
http://purl.uniprot.org/citations/36344519http://purl.uniprot.org/core/pages"6718"xsd:string
http://purl.uniprot.org/citations/36344519http://purl.uniprot.org/core/title"Functionalized graphene grids with various charges for single-particle cryo-EM."xsd:string
http://purl.uniprot.org/citations/36344519http://purl.uniprot.org/core/volume"13"xsd:string
http://purl.uniprot.org/citations/36344519http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/36344519
http://purl.uniprot.org/citations/36344519http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/36344519
http://purl.uniprot.org/uniprot/#_P0A3U0-mappedCitation-36344519http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/36344519
http://purl.uniprot.org/uniprot/P0A3U0http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/36344519