| http://purl.uniprot.org/citations/36546838 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/36546838 | http://www.w3.org/2000/01/rdf-schema#comment | "Mammalian nephrons arise from a population of nephron progenitor cells (NPCs) expressing the master transcription factor Wilms tumor-1 (WT1), which is crucial for NPC proliferation, migration, and differentiation. In humans, biallelic loss of WT1 precludes nephrogenesis and leads to the formation of Wilms tumor precursor lesions. We hypothesize that WT1 normally primes the NPC for nephrogenesis by inducing expression of NPC-specific DNA repair genes that protect the genome. We analyzed transcript levels for a panel of DNA repair genes in embryonic day 17.5 (E17.5) versus adult mouse kidneys and noted seven genes that were increased >20-fold. We then isolated Cited1+ NPCs from E17.5 kidneys and found that only one gene, nei-like DNA glycosylase 3 (Neil3), was enriched. RNAscope in situ hybridization of E17.5 mouse kidneys showed increased Neil3 expression in the nephrogenic zone versus mature nephron structures. To determine whether Neil3 expression is WT1 dependent, we knocked down Wt1 in Cited1+ NPCs (60% knockdown efficiency) and noted a 58% reduction in Neil3 transcript levels. We showed that WT1 interacts with the Neil3 promoter and that activity of a Neil3 promoter-reporter vector was increased twofold in WT1+ versus WT1- cells. We propose that Neil3 is a WT1-dependent DNA repair gene expressed at high levels in Cited1+ NPCs, where it repairs mutational injury to the genome during nephrogenesis. NEIL3 is likely just one of many such lineage-specific repair mechanisms that respond to genomic injury during kidney development.NEW & NOTEWORTHY We studied the molecular events leading to Wilms tumors as a model for the repair of genomic injury. Specifically, we showed that WT1 activates DNA repair gene Neil3 in nephron progenitor cells. However, our observations offer a much broader principle, demonstrating that the embryonic kidney invests in lineage-specific expression of DNA repair enzymes. Thus, it is conceivable that failure of these mechanisms could lead to a variety of "sporadic" congenital renal malformations and human disease."xsd:string |
| http://purl.uniprot.org/citations/36546838 | http://purl.org/dc/terms/identifier | "doi:10.1152/ajprenal.00207.2022"xsd:string |
| http://purl.uniprot.org/citations/36546838 | http://purl.uniprot.org/core/author | "Goodyer P."xsd:string |
| http://purl.uniprot.org/citations/36546838 | http://purl.uniprot.org/core/author | "Hammond L."xsd:string |
| http://purl.uniprot.org/citations/36546838 | http://purl.uniprot.org/core/author | "Dickinson K."xsd:string |
| http://purl.uniprot.org/citations/36546838 | http://purl.uniprot.org/core/author | "Chu L.L."xsd:string |
| http://purl.uniprot.org/citations/36546838 | http://purl.uniprot.org/core/author | "Akpa M."xsd:string |
| http://purl.uniprot.org/citations/36546838 | http://purl.uniprot.org/core/author | "Goumba A."xsd:string |
| http://purl.uniprot.org/citations/36546838 | http://purl.uniprot.org/core/author | "Lalonde C.T."xsd:string |
| http://purl.uniprot.org/citations/36546838 | http://purl.uniprot.org/core/date | "2023"xsd:gYear |
| http://purl.uniprot.org/citations/36546838 | http://purl.uniprot.org/core/name | "Am J Physiol Renal Physiol"xsd:string |
| http://purl.uniprot.org/citations/36546838 | http://purl.uniprot.org/core/pages | "F245-F255"xsd:string |
| http://purl.uniprot.org/citations/36546838 | http://purl.uniprot.org/core/title | "WT1 regulates expression of DNA repair gene Neil3 during nephrogenesis."xsd:string |
| http://purl.uniprot.org/citations/36546838 | http://purl.uniprot.org/core/volume | "324"xsd:string |
| http://purl.uniprot.org/citations/36546838 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/36546838 |
| http://purl.uniprot.org/citations/36546838 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/36546838 |
| http://purl.uniprot.org/uniprot/#_A0A0A6YVY2-mappedCitation-36546838 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/36546838 |
| http://purl.uniprot.org/uniprot/#_A0A0G2JDS8-mappedCitation-36546838 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/36546838 |
| http://purl.uniprot.org/uniprot/#_A0A0A6YWF4-mappedCitation-36546838 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/36546838 |
| http://purl.uniprot.org/uniprot/#_A0A0A6YWI7-mappedCitation-36546838 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/36546838 |
| http://purl.uniprot.org/uniprot/#_A0A0A6YWN5-mappedCitation-36546838 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/36546838 |
| http://purl.uniprot.org/uniprot/#_A0A0A6YWZ8-mappedCitation-36546838 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/36546838 |
| http://purl.uniprot.org/uniprot/#_A0A0A6YX74-mappedCitation-36546838 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/36546838 |
| http://purl.uniprot.org/uniprot/#_A0A0A6YXQ6-mappedCitation-36546838 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/36546838 |