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http://purl.uniprot.org/citations/36707786http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/36707786http://www.w3.org/2000/01/rdf-schema#comment"

Background

Type 2 Diabetes mellitus (T2DM) is a major risk factor for cardiovascular disease and associated with poor outcome after myocardial infarction (MI). In T2DM, cardiac metabolic flexibility, i.e. the switch between carbohydrates and lipids as energy source, is disturbed. The RabGTPase-activating protein TBC1D4 represents a crucial regulator of insulin-stimulated glucose uptake in skeletal muscle by controlling glucose transporter GLUT4 translocation. A human loss-of-function mutation in TBC1D4 is associated with impaired glycemic control and elevated T2DM risk. The study's aim was to investigate TBC1D4 function in cardiac substrate metabolism and adaptation to MI.

Methods

Cardiac glucose metabolism of male Tbc1d4-deficient (D4KO) and wild type (WT) mice was characterized using in vivo [18F]-FDG PET imaging after glucose injection and ex vivo basal/insulin-stimulated [3H]-2-deoxyglucose uptake in left ventricular (LV) papillary muscle. Mice were subjected to cardiac ischemia/reperfusion (I/R). Heart structure and function were analyzed until 3 weeks post-MI using echocardiography, morphometric and ultrastructural analysis of heart sections, complemented by whole heart transcriptome and protein measurements.

Results

Tbc1d4-knockout abolished insulin-stimulated glucose uptake in ex vivo LV papillary muscle and in vivo cardiac glucose uptake after glucose injection, accompanied by a marked reduction of GLUT4. Basal cardiac glucose uptake and GLUT1 abundance were not changed compared to WT controls. D4KO mice showed mild impairments in glycemia but normal cardiac function. However, after I/R D4KO mice showed progressively increased LV endsystolic volume and substantially increased infarction area compared to WT controls. Cardiac transcriptome analysis revealed upregulation of the unfolded protein response via ATF4/eIF2α in D4KO mice at baseline. Transmission electron microscopy revealed largely increased extracellular matrix (ECM) area, in line with decreased cardiac expression of matrix metalloproteinases of D4KO mice.

Conclusions

TBC1D4 is essential for insulin-stimulated cardiac glucose uptake and metabolic flexibility. Tbc1d4-deficiency results in elevated cardiac endoplasmic reticulum (ER)-stress response, increased deposition of ECM and aggravated cardiac damage following MI. Hence, impaired TBC1D4 signaling contributes to poor outcome after MI."xsd:string
http://purl.uniprot.org/citations/36707786http://purl.org/dc/terms/identifier"doi:10.1186/s12933-023-01746-2"xsd:string
http://purl.uniprot.org/citations/36707786http://purl.uniprot.org/core/author"Weiss J."xsd:string
http://purl.uniprot.org/citations/36707786http://purl.uniprot.org/core/author"Herwig R."xsd:string
http://purl.uniprot.org/citations/36707786http://purl.uniprot.org/core/author"Timmermann B."xsd:string
http://purl.uniprot.org/citations/36707786http://purl.uniprot.org/core/author"Al-Hasani H."xsd:string
http://purl.uniprot.org/citations/36707786http://purl.uniprot.org/core/author"Hubert M."xsd:string
http://purl.uniprot.org/citations/36707786http://purl.uniprot.org/core/author"Jeruschke K."xsd:string
http://purl.uniprot.org/citations/36707786http://purl.uniprot.org/core/author"Lienhard M."xsd:string
http://purl.uniprot.org/citations/36707786http://purl.uniprot.org/core/author"Kolasa M."xsd:string
http://purl.uniprot.org/citations/36707786http://purl.uniprot.org/core/author"Fischer J.W."xsd:string
http://purl.uniprot.org/citations/36707786http://purl.uniprot.org/core/author"Springer C."xsd:string
http://purl.uniprot.org/citations/36707786http://purl.uniprot.org/core/author"Chadt A."xsd:string
http://purl.uniprot.org/citations/36707786http://purl.uniprot.org/core/author"Backes H."xsd:string
http://purl.uniprot.org/citations/36707786http://purl.uniprot.org/core/author"Gorressen S."xsd:string
http://purl.uniprot.org/citations/36707786http://purl.uniprot.org/core/author"Borno S."xsd:string
http://purl.uniprot.org/citations/36707786http://purl.uniprot.org/core/author"Binsch C."xsd:string
http://purl.uniprot.org/citations/36707786http://purl.uniprot.org/core/author"Cremer A.L."xsd:string
http://purl.uniprot.org/citations/36707786http://purl.uniprot.org/core/author"Barbosa D.M."xsd:string
http://purl.uniprot.org/citations/36707786http://purl.uniprot.org/core/author"Hansen-Dille G."xsd:string
http://purl.uniprot.org/citations/36707786http://purl.uniprot.org/core/author"Hodge S.M."xsd:string
http://purl.uniprot.org/citations/36707786http://purl.uniprot.org/core/date"2023"xsd:gYear
http://purl.uniprot.org/citations/36707786http://purl.uniprot.org/core/name"Cardiovasc Diabetol"xsd:string
http://purl.uniprot.org/citations/36707786http://purl.uniprot.org/core/pages"17"xsd:string
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