| http://purl.uniprot.org/citations/36739096 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/36739096 | http://www.w3.org/2000/01/rdf-schema#comment | "Selenomethionine (Se-Met) has many beneficial effects on higher animals and human, and can regulate cellular physiology through distinct signaling pathways. However, the role and molecular mechanism of Se-Met in skeletal muscle growth remains unclear. In this study, we observed the effects of Se-Met on C2C12 myoblasts and skeletal muscle growth of mice, and explored the corresponding molecular mechanism. Se-Met affected proliferation and protein synthesis of C2C12 myoblasts in a hormesis type of relationship, and had an optimal stimulatory effect at 50 µM concentration. Se-Met also affected mTOR, ANXA2, and PKCα phosphorylation in the same manner. ANXA2 knockdown blocked the stimulation of Se-Met on cell proliferation and protein synthesis and inhibition of Se-Met on autophagy of C2C12 myoblasts. Western blotting analysis showed that PI3K inhibition blocked the stimulation of Se-Met on mTOR phosphorylation. ANXA2 knockdown further blocked the stimulation of Se-Met on PI3K and mTOR phosphorylation. Point mutation experiment showed that ANXA2 mediated the stimulation of Se-Met on the PI3K-mTOR signaling through phosphorylation at Ser26. PKCα interacted with ANXA2, and PKCα knockdown blocked the stimulation of Se-Met on ANXA2 phosphorylation at Ser26. Se-Met addition (7.5mg/kg diet, 4 weeks) increased mouse carcass weight, promoted gastrocnemius skeletal muscle growth and ANXA2 and mTOR phosphorylation in this tissue. Collectively, our findings reveal that Se-Met can promote proliferation and protein synthesis of myoblasts and skeletal muscle growth through ANXA2 phosphorylation."xsd:string |
| http://purl.uniprot.org/citations/36739096 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.jnutbio.2023.109277"xsd:string |
| http://purl.uniprot.org/citations/36739096 | http://purl.uniprot.org/core/author | "Cui X."xsd:string |
| http://purl.uniprot.org/citations/36739096 | http://purl.uniprot.org/core/author | "Gao X."xsd:string |
| http://purl.uniprot.org/citations/36739096 | http://purl.uniprot.org/core/author | "Ma Z."xsd:string |
| http://purl.uniprot.org/citations/36739096 | http://purl.uniprot.org/core/author | "Li R."xsd:string |
| http://purl.uniprot.org/citations/36739096 | http://purl.uniprot.org/core/author | "Li X."xsd:string |
| http://purl.uniprot.org/citations/36739096 | http://purl.uniprot.org/core/author | "Zhang M."xsd:string |
| http://purl.uniprot.org/citations/36739096 | http://purl.uniprot.org/core/date | "2023"xsd:gYear |
| http://purl.uniprot.org/citations/36739096 | http://purl.uniprot.org/core/name | "J Nutr Biochem"xsd:string |
| http://purl.uniprot.org/citations/36739096 | http://purl.uniprot.org/core/pages | "109277"xsd:string |
| http://purl.uniprot.org/citations/36739096 | http://purl.uniprot.org/core/title | "Selenomethionine promotes ANXA2 phosphorylation for proliferation and protein synthesis of myoblasts and skeletal muscle growth."xsd:string |
| http://purl.uniprot.org/citations/36739096 | http://purl.uniprot.org/core/volume | "115"xsd:string |
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