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http://purl.uniprot.org/citations/36930145http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/36930145http://www.w3.org/2000/01/rdf-schema#comment"

Purpose

Scleritis is a severe inflammatory ocular disorder with unknown pathogenesis. We investigated healthy sclera as well as sclera affected by noninfectious scleritis for differentially expressed proteins using a mass spectrometry approach.

Methods

We collected scleral samples of enucleated eyes due to severe noninfectious scleritis (n = 3), and control scleral tissues (n = 5), all exenterated eyes for eyelid carcinomas (n = 4), or choroidal melanoma (n = 1) without scleral invasion. Samples were prepared for the nano liquid-chromatography mass spectrometer (LC-MS), data were analyzed using proteomics software (Scaffold), and is available via ProteomeXchange (identifier PXD038727). Samples were also stained for immuno-histopathological evaluation.

Results

Mass spectrometry identified 629 proteins within the healthy and diseased scleral tissues, whereof collagen type XII, VI, and I were the most abundantly expressed protein. Collagen type II-XII was also present. Filaggrin-2, a protein that plays a crucial role in epidermal barrier function, was found upregulated in all scleritis cases. In addition, other epithelial associated proteins were upregulated (such as keratin 33b, 34, and 85, epiplakin, transglutaminase-3, galectin 7, and caspase-14) in scleritis. Further, upregulated proteins involved in regulation of the cytoskeleton (vinculin and myosin 9), and housekeeping proteins were found (elongation factor-2 and cytoplasmic dynein 1) in our study. Upregulation of filaggrin-2 and myosin-9 was confirmed with immunohistochemistry, the latter protein showing co-localization with the endothelial cell marker ETC-related gene (ERG), indicating neovascularization in scleral tissue affected by scleritis.

Conclusions

We found upregulation of filaggrin-2 and signs of neovascularization in scleral tissue of patients with noninfectious scleritis. Further research, ideally including more scleritis cases, is needed to validate our findings."xsd:string
http://purl.uniprot.org/citations/36930145http://purl.org/dc/terms/identifier"doi:10.1167/iovs.64.3.27"xsd:string
http://purl.uniprot.org/citations/36930145http://purl.uniprot.org/core/author"Verdijk R.M."xsd:string
http://purl.uniprot.org/citations/36930145http://purl.uniprot.org/core/author"Luider T.M."xsd:string
http://purl.uniprot.org/citations/36930145http://purl.uniprot.org/core/author"Schreurs M.W.J."xsd:string
http://purl.uniprot.org/citations/36930145http://purl.uniprot.org/core/author"Rothova A."xsd:string
http://purl.uniprot.org/citations/36930145http://purl.uniprot.org/core/author"Guzel C."xsd:string
http://purl.uniprot.org/citations/36930145http://purl.uniprot.org/core/author"van den Bosch T.P.P."xsd:string
http://purl.uniprot.org/citations/36930145http://purl.uniprot.org/core/author"Ten Berge J.C."xsd:string
http://purl.uniprot.org/citations/36930145http://purl.uniprot.org/core/author"Vergouwen D.P.C."xsd:string
http://purl.uniprot.org/citations/36930145http://purl.uniprot.org/core/date"2023"xsd:gYear
http://purl.uniprot.org/citations/36930145http://purl.uniprot.org/core/name"Invest Ophthalmol Vis Sci"xsd:string
http://purl.uniprot.org/citations/36930145http://purl.uniprot.org/core/pages"27"xsd:string
http://purl.uniprot.org/citations/36930145http://purl.uniprot.org/core/title"Scleral Proteome in Noninfectious Scleritis Unravels Upregulation of Filaggrin-2 and Signs of Neovascularization."xsd:string
http://purl.uniprot.org/citations/36930145http://purl.uniprot.org/core/volume"64"xsd:string
http://purl.uniprot.org/citations/36930145http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/36930145
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http://purl.uniprot.org/uniprot/#_L8E7D3-mappedCitation-36930145http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/36930145
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