| http://purl.uniprot.org/citations/36951092 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/36951092 | http://www.w3.org/2000/01/rdf-schema#comment | "Posttranscriptional regulation of the maternal nanos mRNA is essential for the development of the anterior - posterior axis of the Drosophila embryo. The nanos RNA is regulated by the protein Smaug, which binds to Smaug recognition elements (SREs) in the nanos 3'-UTR and nucleates the assembly of a larger repressor complex including the eIF4E-T paralog Cup and five additional proteins. The Smaug-dependent complex represses translation of nanos and induces its deadenylation by the CCR4-NOT deadenylase. Here we report an in vitro reconstitution of the Drosophila CCR4-NOT complex and Smaug-dependent deadenylation. We find that Smaug by itself is sufficient to cause deadenylation by the Drosophila or human CCR4-NOT complexes in an SRE-dependent manner. CCR4-NOT subunits NOT10 and NOT11 are dispensable, but the NOT module, consisting of NOT2, NOT3 and the C-terminal part of NOT1, is required. Smaug interacts with the C-terminal domain of NOT3. Both catalytic subunits of CCR4-NOT contribute to Smaug-dependent deadenylation. Whereas the CCR4-NOT complex itself acts distributively, Smaug induces a processive behavior. The cytoplasmic poly(A) binding protein (PABPC) has a minor inhibitory effect on Smaug-dependent deadenylation. Among the additional constituents of the Smaug-dependent repressor complex, Cup also facilitates CCR4-NOT-dependent deadenylation, both independently and in cooperation with Smaug."xsd:string |
| http://purl.uniprot.org/citations/36951092 | http://purl.org/dc/terms/identifier | "doi:10.1093/nar/gkad159"xsd:string |
| http://purl.uniprot.org/citations/36951092 | http://purl.uniprot.org/core/author | "Jeske M."xsd:string |
| http://purl.uniprot.org/citations/36951092 | http://purl.uniprot.org/core/author | "Wahle E."xsd:string |
| http://purl.uniprot.org/citations/36951092 | http://purl.uniprot.org/core/author | "Metz J."xsd:string |
| http://purl.uniprot.org/citations/36951092 | http://purl.uniprot.org/core/author | "Rammelt C."xsd:string |
| http://purl.uniprot.org/citations/36951092 | http://purl.uniprot.org/core/author | "Pekovic F."xsd:string |
| http://purl.uniprot.org/citations/36951092 | http://purl.uniprot.org/core/author | "Kubikova J."xsd:string |
| http://purl.uniprot.org/citations/36951092 | http://purl.uniprot.org/core/date | "2023"xsd:gYear |
| http://purl.uniprot.org/citations/36951092 | http://purl.uniprot.org/core/name | "Nucleic Acids Res"xsd:string |
| http://purl.uniprot.org/citations/36951092 | http://purl.uniprot.org/core/pages | "3950-3970"xsd:string |
| http://purl.uniprot.org/citations/36951092 | http://purl.uniprot.org/core/title | "RNA binding proteins Smaug and Cup induce CCR4-NOT-dependent deadenylation of the nanos mRNA in a reconstituted system."xsd:string |
| http://purl.uniprot.org/citations/36951092 | http://purl.uniprot.org/core/volume | "51"xsd:string |
| http://purl.uniprot.org/citations/36951092 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/36951092 |
| http://purl.uniprot.org/citations/36951092 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/36951092 |
| http://purl.uniprot.org/uniprot/#_A0A0B4LEZ3-mappedCitation-36951092 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/36951092 |
| http://purl.uniprot.org/uniprot/#_A0A0B4KGU4-mappedCitation-36951092 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/36951092 |
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| http://purl.uniprot.org/uniprot/#_A0A0B4LHS9-mappedCitation-36951092 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/36951092 |
| http://purl.uniprot.org/uniprot/#_A0A6M3Q6V5-mappedCitation-36951092 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/36951092 |
| http://purl.uniprot.org/uniprot/#_A0A126GUU0-mappedCitation-36951092 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/36951092 |
| http://purl.uniprot.org/uniprot/#_A8DY80-mappedCitation-36951092 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/36951092 |
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