| http://purl.uniprot.org/citations/37025950 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/37025950 | http://www.w3.org/2000/01/rdf-schema#comment | "DNA transposon-based gene delivery vectors represent a promising new branch of randomly integrating vector development for gene therapy. For the side-by-side evaluation of the piggyBac and Sleeping Beauty systems-the only DNA transposons currently employed in clinical trials-during therapeutic intervention, we treated the mouse model of tyrosinemia type I with liver-targeted gene delivery using both transposon vectors. For genome-wide mapping of transposon insertion sites we developed a new next-generation sequencing procedure called streptavidin-based enrichment sequencing, which allowed us to identify approximately one million integration sites for both systems. We revealed that a high proportion of piggyBac integrations are clustered in hot regions and found that they are frequently recurring at the same genomic positions among treated animals, indicating that the genome-wide distribution of Sleeping Beauty-generated integrations is closer to random. We also revealed that the piggyBac transposase protein exhibits prolonged activity, which predicts the risk of oncogenesis by generating chromosomal double-strand breaks. Safety concerns associated with prolonged transpositional activity draw attention to the importance of squeezing the active state of the transposase enzymes into a narrower time window."xsd:string |
| http://purl.uniprot.org/citations/37025950 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.omtm.2023.03.003"xsd:string |
| http://purl.uniprot.org/citations/37025950 | http://purl.uniprot.org/core/author | "Nagy I."xsd:string |
| http://purl.uniprot.org/citations/37025950 | http://purl.uniprot.org/core/author | "Takacs B."xsd:string |
| http://purl.uniprot.org/citations/37025950 | http://purl.uniprot.org/core/author | "Kovacs A."xsd:string |
| http://purl.uniprot.org/citations/37025950 | http://purl.uniprot.org/core/author | "Nagy L.G."xsd:string |
| http://purl.uniprot.org/citations/37025950 | http://purl.uniprot.org/core/author | "Nagy A."xsd:string |
| http://purl.uniprot.org/citations/37025950 | http://purl.uniprot.org/core/author | "Balint B."xsd:string |
| http://purl.uniprot.org/citations/37025950 | http://purl.uniprot.org/core/author | "Haracska L."xsd:string |
| http://purl.uniprot.org/citations/37025950 | http://purl.uniprot.org/core/author | "Rulicke T."xsd:string |
| http://purl.uniprot.org/citations/37025950 | http://purl.uniprot.org/core/author | "Barta E."xsd:string |
| http://purl.uniprot.org/citations/37025950 | http://purl.uniprot.org/core/author | "Vasarhelyi B.M."xsd:string |
| http://purl.uniprot.org/citations/37025950 | http://purl.uniprot.org/core/author | "Mates L."xsd:string |
| http://purl.uniprot.org/citations/37025950 | http://purl.uniprot.org/core/author | "Blastyak A."xsd:string |
| http://purl.uniprot.org/citations/37025950 | http://purl.uniprot.org/core/author | "Hegedus Z."xsd:string |
| http://purl.uniprot.org/citations/37025950 | http://purl.uniprot.org/core/author | "Latinovics D."xsd:string |
| http://purl.uniprot.org/citations/37025950 | http://purl.uniprot.org/core/author | "German P."xsd:string |
| http://purl.uniprot.org/citations/37025950 | http://purl.uniprot.org/core/author | "Sukosd F."xsd:string |
| http://purl.uniprot.org/citations/37025950 | http://purl.uniprot.org/core/author | "Imre G."xsd:string |
| http://purl.uniprot.org/citations/37025950 | http://purl.uniprot.org/core/author | "Pinter L."xsd:string |
| http://purl.uniprot.org/citations/37025950 | http://purl.uniprot.org/core/author | "Pankotai-Bodo G."xsd:string |
| http://purl.uniprot.org/citations/37025950 | http://purl.uniprot.org/core/author | "Jaksa G."xsd:string |
| http://purl.uniprot.org/citations/37025950 | http://purl.uniprot.org/core/author | "Drubi A.B."xsd:string |
| http://purl.uniprot.org/citations/37025950 | http://purl.uniprot.org/core/author | "Hudoba L."xsd:string |