| http://purl.uniprot.org/citations/37058288 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/37058288 | http://www.w3.org/2000/01/rdf-schema#comment | "Lysosomal integrity is vital for cell homeostasis, but the underlying mechanisms are poorly understood. Here, we identify CLH-6, the C. elegans ortholog of the lysosomal Cl-/H+ antiporter ClC-7, as an important factor for protecting lysosomal integrity. Loss of CLH-6 affects lysosomal degradation, causing cargo accumulation and membrane rupture. Reducing cargo delivery or increasing CPL-1/cathepsin L or CPR-2/cathepsin B expression suppresses these lysosomal defects. Inactivation of CPL-1 or CPR-2, like CLH-6 inactivation, affects cargo digestion and causes lysosomal membrane rupture. Thus, loss of CLH-6 impairs cargo degradation, leading to membrane damage of lysosomes. In clh-6(lf) mutants, lysosomes are acidified as in wild type but contain lower chloride levels, and cathepsin B and L activities are significantly reduced. Cl-binds to CPL-1 and CPR-2 in vitro, and Cl-supplementation increases lysosomal cathepsin B and L activities. Altogether, these findings suggest that CLH-6 maintains the luminal chloride levels required for cathepsin activity, thus facilitating substrate digestion to protect lysosomal membrane integrity."xsd:string |
| http://purl.uniprot.org/citations/37058288 | http://purl.org/dc/terms/identifier | "doi:10.1083/jcb.202210063"xsd:string |
| http://purl.uniprot.org/citations/37058288 | http://purl.uniprot.org/core/author | "Li Y."xsd:string |
| http://purl.uniprot.org/citations/37058288 | http://purl.uniprot.org/core/author | "Li M."xsd:string |
| http://purl.uniprot.org/citations/37058288 | http://purl.uniprot.org/core/author | "Zhang Q."xsd:string |
| http://purl.uniprot.org/citations/37058288 | http://purl.uniprot.org/core/author | "Wang X."xsd:string |
| http://purl.uniprot.org/citations/37058288 | http://purl.uniprot.org/core/author | "Jian Y."xsd:string |
| http://purl.uniprot.org/citations/37058288 | http://purl.uniprot.org/core/date | "2023"xsd:gYear |
| http://purl.uniprot.org/citations/37058288 | http://purl.uniprot.org/core/name | "J Cell Biol"xsd:string |
| http://purl.uniprot.org/citations/37058288 | http://purl.uniprot.org/core/pages | "e202210063"xsd:string |
| http://purl.uniprot.org/citations/37058288 | http://purl.uniprot.org/core/title | "Lysosomal chloride transporter CLH-6 protects lysosome membrane integrity via cathepsin activation."xsd:string |
| http://purl.uniprot.org/citations/37058288 | http://purl.uniprot.org/core/volume | "222"xsd:string |
| http://purl.uniprot.org/citations/37058288 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/37058288 |
| http://purl.uniprot.org/citations/37058288 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/37058288 |
| http://purl.uniprot.org/uniprot/#_G5EC75-mappedCitation-37058288 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/37058288 |
| http://purl.uniprot.org/uniprot/G5EC75 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/37058288 |