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http://purl.uniprot.org/citations/37094862http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/37094862http://www.w3.org/2000/01/rdf-schema#comment"

Objective

Abdominal aortic aneurysm (AAA) is characterized by vascular smooth muscle cell (VSMC) injury. Circ_0000285 has been declared to drive cancer development, but its role in AAA remains unclear. We thus intended to disclose circ_0000285's role and molecular mechanism in AAA.

Methods

VSMCs were exposed to hydrogen peroxide (H2O2) to induce cell injury. Circ_0000285, miR-599, and regulator of G protein signaling 17 (RGS17) mRNA expressions were ascertained by conducting RT-qPCR assay while the levels of RGS17 protein was ascertained via western blotting. MiR-599's predicted binding with circ_0000285 and RGS17 were validated by means of the dual-luciferase reporter experiment. Cell proliferation was evaluated through the CCK-8 and EdU assays. Cell apoptosis was assessed via the caspase-3 activity assay.

Results

The AAA samples and H2O2-treated VSMCs manifested high expressions of circ_0000285 and RGS17 as well as a poor miR-599 expression. H2O2 treatment impaired the proliferation of VSMCs while stimulating their apoptosis. Circ_0000285 overexpression further repressed cell proliferation and enhanced apoptosis in H2O2-treated VSMCs while miR-599 enrichment partly reversed these effects. Circ_0000285 directly bound to miR-599, and miR-599 interacted with RGS17 3'UTR. RGS17 overexpression also suppressed cell proliferation and stimulated apoptosis in H2O2-treated VSMCs. Nevertheless, these effects were offset by miR-599 enrichment.

Conclusion

Circ_0000285 governed the miR-599/RGS17 network to regulate H2O2-induced VSMC injuries, thereby promoting the development of AAA."xsd:string
http://purl.uniprot.org/citations/37094862http://purl.uniprot.org/core/author"Liu Y."xsd:string
http://purl.uniprot.org/citations/37094862http://purl.uniprot.org/core/author"Ren J."xsd:string
http://purl.uniprot.org/citations/37094862http://purl.uniprot.org/core/author"Xie P."xsd:string
http://purl.uniprot.org/citations/37094862http://purl.uniprot.org/core/author"Fang C."xsd:string
http://purl.uniprot.org/citations/37094862http://purl.uniprot.org/core/date"2023"xsd:gYear
http://purl.uniprot.org/citations/37094862http://purl.uniprot.org/core/name"Ann Clin Lab Sci"xsd:string
http://purl.uniprot.org/citations/37094862http://purl.uniprot.org/core/pages"238-247"xsd:string
http://purl.uniprot.org/citations/37094862http://purl.uniprot.org/core/title"Hsa_circ_0000285-Mediated miR-599/RGS17 Axis Participates in the Pathogenesis of Abdominal Aortic Aneurysm by Regulating the Functions of Vascular Smooth Muscle Cells."xsd:string
http://purl.uniprot.org/citations/37094862http://purl.uniprot.org/core/volume"53"xsd:string
http://purl.uniprot.org/citations/37094862http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/37094862
http://purl.uniprot.org/citations/37094862http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/37094862
http://purl.uniprot.org/uniprot/#_Q9UGC6-mappedCitation-37094862http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/37094862
http://purl.uniprot.org/uniprot/Q9UGC6http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/37094862