| http://purl.uniprot.org/citations/37308707 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/37308707 | http://www.w3.org/2000/01/rdf-schema#comment | "As genetic code expansion advances beyond L-α-amino acids to backbone modifications and new polymerization chemistries, delineating what substrates the ribosome can accommodate remains a challenge. The Escherichia coli ribosome tolerates non-L-α-amino acids in vitro, but few structural insights that explain how are available, and the boundary conditions for efficient bond formation are so far unknown. Here we determine a high-resolution cryogenic electron microscopy structure of the E. coli ribosome containing α-amino acid monomers and use metadynamics simulations to define energy surface minima and understand incorporation efficiencies. Reactive monomers across diverse structural classes favour a conformational space where the aminoacyl-tRNA nucleophile is <4 Å from the peptidyl-tRNA carbonyl with a Bürgi-Dunitz angle of 76-115°. Monomers with free energy minima that fall outside this conformational space do not react efficiently. This insight should accelerate the in vivo and in vitro ribosomal synthesis of sequence-defined, non-peptide heterooligomers."xsd:string |
| http://purl.uniprot.org/citations/37308707 | http://purl.org/dc/terms/identifier | "doi:10.1038/s41557-023-01226-w"xsd:string |
| http://purl.uniprot.org/citations/37308707 | http://purl.uniprot.org/core/author | "Cate J.H.D."xsd:string |
| http://purl.uniprot.org/citations/37308707 | http://purl.uniprot.org/core/author | "Miller S.J."xsd:string |
| http://purl.uniprot.org/citations/37308707 | http://purl.uniprot.org/core/author | "Schepartz A."xsd:string |
| http://purl.uniprot.org/citations/37308707 | http://purl.uniprot.org/core/author | "Watson Z.L."xsd:string |
| http://purl.uniprot.org/citations/37308707 | http://purl.uniprot.org/core/author | "Ward F.R."xsd:string |
| http://purl.uniprot.org/citations/37308707 | http://purl.uniprot.org/core/author | "Abramyan A.M."xsd:string |
| http://purl.uniprot.org/citations/37308707 | http://purl.uniprot.org/core/author | "Knudson I.J."xsd:string |
| http://purl.uniprot.org/citations/37308707 | http://purl.uniprot.org/core/date | "2023"xsd:gYear |
| http://purl.uniprot.org/citations/37308707 | http://purl.uniprot.org/core/name | "Nat Chem"xsd:string |
| http://purl.uniprot.org/citations/37308707 | http://purl.uniprot.org/core/pages | "913-921"xsd:string |
| http://purl.uniprot.org/citations/37308707 | http://purl.uniprot.org/core/title | "Atomistic simulations of the Escherichia coli ribosome provide selection criteria for translationally active substrates."xsd:string |
| http://purl.uniprot.org/citations/37308707 | http://purl.uniprot.org/core/volume | "15"xsd:string |
| http://purl.uniprot.org/citations/37308707 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/37308707 |
| http://purl.uniprot.org/citations/37308707 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/37308707 |
| http://purl.uniprot.org/uniprot/#_P0ADZ0-mappedCitation-37308707 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/37308707 |
| http://purl.uniprot.org/uniprot/#_P0ADZ4-mappedCitation-37308707 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/37308707 |
| http://purl.uniprot.org/uniprot/#_P0AG44-mappedCitation-37308707 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/37308707 |
| http://purl.uniprot.org/uniprot/#_P0AG48-mappedCitation-37308707 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/37308707 |
| http://purl.uniprot.org/uniprot/#_P0AG51-mappedCitation-37308707 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/37308707 |
| http://purl.uniprot.org/uniprot/#_P0AG55-mappedCitation-37308707 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/37308707 |
| http://purl.uniprot.org/uniprot/#_P0AG59-mappedCitation-37308707 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/37308707 |
| http://purl.uniprot.org/uniprot/#_P0AG63-mappedCitation-37308707 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/37308707 |