| http://purl.uniprot.org/citations/37384578 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/37384578 | http://www.w3.org/2000/01/rdf-schema#comment | "Strigolactones (SLs) play fundamental roles in regulating plant architecture, which is a major factor determining crop yield. The perception and signal transduction of SLs require the formation of a complex containing the receptor DWARF14 (D14), an F-box protein D3 and a transcriptional regulator D53 in an SL-dependent manner. Structural and biochemical analyses of D14 and its orthologs DAD2 and AtD14, D3 and the complexes of ASK1-D3-AtD14 and D3CTH-D14 have made great contributions to understanding the mechanisms of SL perception. However, structural analyses of D53 and the D53-D3-D14 holo-complex are challenging, and the biochemical mechanism underlying the complex assembly remains poorly understood. Here, we found that apo-D53 was rather flexible and reconstituted the holo-complex containing D53, S-phase kinase-associated protein 1 (SKP1), D3 and D14 with rac-GR24. The cryo-electron microscopy (cryo-EM) structure of SKP1-D3-D14 in the presence of D53 was analyzed and superimposed on the crystal structure of ASK1-D3-AtD14 without D53. No large conformational rearrangement was observed, but a 9Å rotation appeared between D14 and AtD14. Using hydrogen-deuterium exchange monitored by mass spectrometry, we analyzed dynamic motifs of D14, D3 and D53 in the D53-SKP1-D3-D14 complex assembly process and further identified two potential interfaces in D53 that are located in the N and D2 domains, respectively. Together, our results uncovered the dynamic conformational changes and built a model of the holo-complex D53-SKP1-D3-D14, offering valuable information for the biochemical and genetic mechanisms of SL perception and signal transduction."xsd:string |
| http://purl.uniprot.org/citations/37384578 | http://purl.org/dc/terms/identifier | "doi:10.1093/pcp/pcad067"xsd:string |
| http://purl.uniprot.org/citations/37384578 | http://purl.uniprot.org/core/author | "Hu Q."xsd:string |
| http://purl.uniprot.org/citations/37384578 | http://purl.uniprot.org/core/author | "Liu H."xsd:string |
| http://purl.uniprot.org/citations/37384578 | http://purl.uniprot.org/core/author | "Li J."xsd:string |
| http://purl.uniprot.org/citations/37384578 | http://purl.uniprot.org/core/author | "Liu S."xsd:string |
| http://purl.uniprot.org/citations/37384578 | http://purl.uniprot.org/core/author | "Gong X."xsd:string |
| http://purl.uniprot.org/citations/37384578 | http://purl.uniprot.org/core/author | "Li Q."xsd:string |
| http://purl.uniprot.org/citations/37384578 | http://purl.uniprot.org/core/author | "Song B."xsd:string |
| http://purl.uniprot.org/citations/37384578 | http://purl.uniprot.org/core/author | "Wang J."xsd:string |
| http://purl.uniprot.org/citations/37384578 | http://purl.uniprot.org/core/author | "Zhang J."xsd:string |
| http://purl.uniprot.org/citations/37384578 | http://purl.uniprot.org/core/author | "Wang H."xsd:string |
| http://purl.uniprot.org/citations/37384578 | http://purl.uniprot.org/core/author | "Wang B."xsd:string |
| http://purl.uniprot.org/citations/37384578 | http://purl.uniprot.org/core/author | "Zheng J."xsd:string |
| http://purl.uniprot.org/citations/37384578 | http://purl.uniprot.org/core/author | "Xu H.E."xsd:string |
| http://purl.uniprot.org/citations/37384578 | http://purl.uniprot.org/core/date | "2023"xsd:gYear |
| http://purl.uniprot.org/citations/37384578 | http://purl.uniprot.org/core/name | "Plant Cell Physiol"xsd:string |
| http://purl.uniprot.org/citations/37384578 | http://purl.uniprot.org/core/pages | "1046-1056"xsd:string |
| http://purl.uniprot.org/citations/37384578 | http://purl.uniprot.org/core/title | "Conformational Dynamics of the D53-D3-D14 Complex in Strigolactone Signaling."xsd:string |
| http://purl.uniprot.org/citations/37384578 | http://purl.uniprot.org/core/volume | "64"xsd:string |
| http://purl.uniprot.org/citations/37384578 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/37384578 |
| http://purl.uniprot.org/citations/37384578 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/37384578 |
| http://purl.uniprot.org/uniprot/#_Q10QA5-mappedCitation-37384578 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/37384578 |
| http://purl.uniprot.org/uniprot/#_Q5VMP0-mappedCitation-37384578 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/37384578 |