| http://purl.uniprot.org/citations/37431588 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/37431588 | http://www.w3.org/2000/01/rdf-schema#comment | "Failure of the lung's endothelial barrier underlies lung injury, which causes the high mortality acute respiratory distress syndrome (ARDS). Multiple organ failure predisposes to the mortality, but mechanisms are poorly understood. Here, we show that mitochondrial uncoupling protein 2 (UCP2), a component of the mitochondrial inner membrane, plays a role in the barrier failure. Subsequent lung-liver cross talk mediated by neutrophil activation causes liver congestion. We intranasally instilled lipopolysaccharide (LPS). Then, we viewed the lung endothelium by real-time confocal imaging of the isolated, blood-perfused mouse lung. LPS caused alveolar-capillary transfer of reactive oxygen species and mitochondrial depolarization in lung venular capillaries. The mitochondrial depolarization was inhibited by transfection of alveolar Catalase and vascular knockdown of UCP2. LPS instillation caused lung injury as indicated by increases in bronchoalveolar lavage (BAL) protein content and extravascular lung water. LPS or Pseudomonas aeruginosa instillation also caused liver congestion, quantified by liver hemoglobin and plasma aspartate aminotransferase (AST) increases. Genetic inhibition of vascular UCP2 prevented both lung injury and liver congestion. Antibody-mediated neutrophil depletion blocked the liver responses, but not lung injury. Knockdown of lung vascular UCP2 mitigated P. aeruginosa-induced mortality. Together, these data suggest a mechanism in which bacterial pneumonia induces oxidative signaling to lung venular capillaries, known sites of inflammatory signaling in the lung microvasculature, depolarizing venular mitochondria. Successive activation of neutrophils induces liver congestion. We conclude that oxidant-induced UCP2 expression in lung venular capillaries causes a mechanistic sequence leading to liver congestion and mortality. Lung vascular UCP2 may present a therapeutic target in ARDS.NEW & NOTEWORTHY We report that mitochondrial injury in lung venular capillaries underlies barrier failure in pneumonia, and venular capillary uncoupling protein 2 (UCP2) causes neutrophil-mediated liver congestion. Using in situ imaging, we found that epithelial-endothelial transfer of H2O2 activates UCP2, depolarizing mitochondria in venular capillaries. The conceptual advance from our findings is that mitochondrial depolarization in lung capillaries mediates liver cross talk through circulating neutrophils. Pharmacologic blockade of UCP2 could be a therapeutic strategy for lung injury."xsd:string |
| http://purl.uniprot.org/citations/37431588 | http://purl.org/dc/terms/identifier | "doi:10.1152/ajplung.00209.2022"xsd:string |
| http://purl.uniprot.org/citations/37431588 | http://purl.uniprot.org/core/author | "Bhattacharya J."xsd:string |
| http://purl.uniprot.org/citations/37431588 | http://purl.uniprot.org/core/author | "Lee M.J."xsd:string |
| http://purl.uniprot.org/citations/37431588 | http://purl.uniprot.org/core/author | "Hough R.F."xsd:string |
| http://purl.uniprot.org/citations/37431588 | http://purl.uniprot.org/core/author | "Emin M.T."xsd:string |
| http://purl.uniprot.org/citations/37431588 | http://purl.uniprot.org/core/date | "2023"xsd:gYear |
| http://purl.uniprot.org/citations/37431588 | http://purl.uniprot.org/core/name | "Am J Physiol Lung Cell Mol Physiol"xsd:string |
| http://purl.uniprot.org/citations/37431588 | http://purl.uniprot.org/core/pages | "L277-L287"xsd:string |
| http://purl.uniprot.org/citations/37431588 | http://purl.uniprot.org/core/title | "Mitochondria of lung venular capillaries mediate lung-liver cross talk in pneumonia."xsd:string |
| http://purl.uniprot.org/citations/37431588 | http://purl.uniprot.org/core/volume | "325"xsd:string |
| http://purl.uniprot.org/citations/37431588 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/37431588 |
| http://purl.uniprot.org/citations/37431588 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/37431588 |
| http://purl.uniprot.org/uniprot/#_A0A140LHA5-mappedCitation-37431588 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/37431588 |
| http://purl.uniprot.org/uniprot/#_D3YXM5-mappedCitation-37431588 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/37431588 |
| http://purl.uniprot.org/uniprot/#_P70406-mappedCitation-37431588 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/37431588 |
| http://purl.uniprot.org/uniprot/#_D3YZE5-mappedCitation-37431588 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/37431588 |
| http://purl.uniprot.org/uniprot/#_D3YZG5-mappedCitation-37431588 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/37431588 |
| http://purl.uniprot.org/uniprot/#_Q549J5-mappedCitation-37431588 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/37431588 |
| http://purl.uniprot.org/uniprot/#_Q8CBU0-mappedCitation-37431588 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/37431588 |
| http://purl.uniprot.org/uniprot/#_Q9R246-mappedCitation-37431588 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/37431588 |
| http://purl.uniprot.org/uniprot/Q9R246 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/37431588 |
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| http://purl.uniprot.org/uniprot/P70406 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/37431588 |