| http://purl.uniprot.org/citations/37549780 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/37549780 | http://www.w3.org/2000/01/rdf-schema#comment | "An active form of vitamin D3 (1,25-dihydroxyvitamin D3) acts through vitamin D receptor (VDR) initiating genomic response, but several studies described also non-genomic actions of 1,25-dihydroxyvitamin D3, implying the role of PDIA3 in the process. PDIA3 is a membrane-associated disulfide isomerase involved in disulfide bond formation, protein folding, and remodeling. Here, we used a transcriptome-based approach to identify changes in expression profiles in PDIA3-deficient squamous cell carcinoma line A431 after 1,25-dihydroxyvitamin D3 treatment. PDIA3 knockout led to changes in the expression of more than 2000 genes and modulated proliferation, cell cycle, and mobility of cells; suggesting an important regulatory role of PDIA3. PDIA3-deficient cells showed increased sensitivity to 1,25-dihydroxyvitamin D3, which led to decrease migration. 1,25-dihydroxyvitamin D3 treatment altered also genes expression profile of A431ΔPDIA3 in comparison to A431WT cells, indicating the existence of PDIA3-dependent genes. Interestingly, classic targets of VDR, including CAMP (Cathelicidin Antimicrobial Peptide), TRPV6 (Transient Receptor Potential Cation Channel Subfamily V Member 6), were regulated differently by 1,25-dihydroxyvitamin D3, in A431ΔPDIA3. Deletion of PDIA3 impaired 1,25-dihydroxyvitamin D3-response of genes, such as PTGS2, MMP12, and FOCAD, which were identified as PDIA3-dependent. Additionally, response to 1,25-dihydroxyvitamin D3 in cancerous A431 cells differed from immortalized HaCaT keratinocytes, used as non-cancerous control. Finally, silencing of PDIA3 and 1,25-dihydroxyvitamin D3, at least partially reverse the expression of cancer-related genes in A431 cells, thus targeting PDIA3 and use of 1,25-dihydroxyvitamin D3 could be considered in a prevention and therapy of the skin cancer. Taken together, PDIA3 has a strong impact on gene expression and physiology, including genomic response to 1,25-dihydroxyvitamin D3."xsd:string |
| http://purl.uniprot.org/citations/37549780 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.steroids.2023.109288"xsd:string |
| http://purl.uniprot.org/citations/37549780 | http://purl.uniprot.org/core/author | "Olszewska A.M."xsd:string |
| http://purl.uniprot.org/citations/37549780 | http://purl.uniprot.org/core/author | "Myszczynski K."xsd:string |
| http://purl.uniprot.org/citations/37549780 | http://purl.uniprot.org/core/author | "Zmijewski M.A."xsd:string |
| http://purl.uniprot.org/citations/37549780 | http://purl.uniprot.org/core/author | "Piotrowska A."xsd:string |
| http://purl.uniprot.org/citations/37549780 | http://purl.uniprot.org/core/author | "Domzalski P."xsd:string |
| http://purl.uniprot.org/citations/37549780 | http://purl.uniprot.org/core/author | "Nowak J.I."xsd:string |
| http://purl.uniprot.org/citations/37549780 | http://purl.uniprot.org/core/date | "2023"xsd:gYear |
| http://purl.uniprot.org/citations/37549780 | http://purl.uniprot.org/core/name | "Steroids"xsd:string |
| http://purl.uniprot.org/citations/37549780 | http://purl.uniprot.org/core/pages | "109288"xsd:string |
| http://purl.uniprot.org/citations/37549780 | http://purl.uniprot.org/core/title | "PDIA3 modulates genomic response to 1,25-dihydroxyvitamin D3 in squamous cell carcinoma of the skin."xsd:string |
| http://purl.uniprot.org/citations/37549780 | http://purl.uniprot.org/core/volume | "199"xsd:string |
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