| http://purl.uniprot.org/citations/37592181 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/37592181 | http://www.w3.org/2000/01/rdf-schema#comment | "Single-particle cryogenic electron microscopy (cryo-EM) allows reconstruction of high-resolution structures of proteins in different conformations. Protein function often involves transient functional conformations, which can be resolved using time-resolved cryo-EM (trEM). In trEM, reactions are arrested after a defined delay time by rapid vitrification of protein solution on the EM grid. Despite the increasing interest in trEM among the cryo-EM community, making trEM samples with a time resolution below 100 ms remains challenging. Here we report the design and the realization of a time-resolved cryo-plunger that combines a droplet-based microfluidic mixer with a laser-induced generator of microjets that allows rapid reaction initiation and plunge-freezing of cryo-EM grids. Using this approach, a time resolution of 5 ms was achieved and the protein density map was reconstructed to a resolution of 2.1 Å. trEM experiments on GroEL:GroES chaperonin complex resolved the kinetics of the complex formation and visualized putative short-lived conformations of GroEL-ATP complex."xsd:string |
| http://purl.uniprot.org/citations/37592181 | http://purl.org/dc/terms/identifier | "doi:10.1038/s41592-023-01967-z"xsd:string |
| http://purl.uniprot.org/citations/37592181 | http://purl.uniprot.org/core/author | "Efremov R.G."xsd:string |
| http://purl.uniprot.org/citations/37592181 | http://purl.uniprot.org/core/author | "Stroobants A."xsd:string |
| http://purl.uniprot.org/citations/37592181 | http://purl.uniprot.org/core/author | "Dhurandhar M."xsd:string |
| http://purl.uniprot.org/citations/37592181 | http://purl.uniprot.org/core/author | "Claessens R."xsd:string |
| http://purl.uniprot.org/citations/37592181 | http://purl.uniprot.org/core/author | "Torino S."xsd:string |
| http://purl.uniprot.org/citations/37592181 | http://purl.uniprot.org/core/date | "2023"xsd:gYear |
| http://purl.uniprot.org/citations/37592181 | http://purl.uniprot.org/core/name | "Nat Methods"xsd:string |
| http://purl.uniprot.org/citations/37592181 | http://purl.uniprot.org/core/pages | "1400-1408"xsd:string |
| http://purl.uniprot.org/citations/37592181 | http://purl.uniprot.org/core/title | "Time-resolved cryo-EM using a combination of droplet microfluidics with on-demand jetting."xsd:string |
| http://purl.uniprot.org/citations/37592181 | http://purl.uniprot.org/core/volume | "20"xsd:string |
| http://purl.uniprot.org/citations/37592181 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/37592181 |
| http://purl.uniprot.org/citations/37592181 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/37592181 |
| http://purl.uniprot.org/uniprot/#_P00722-mappedCitation-37592181 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/37592181 |
| http://purl.uniprot.org/uniprot/#_P09528-mappedCitation-37592181 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/37592181 |
| http://purl.uniprot.org/uniprot/#_P0A6F5-mappedCitation-37592181 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/37592181 |
| http://purl.uniprot.org/uniprot/#_P0A6F9-mappedCitation-37592181 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/37592181 |
| http://purl.uniprot.org/uniprot/P0A6F5 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/37592181 |
| http://purl.uniprot.org/uniprot/P09528 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/37592181 |
| http://purl.uniprot.org/uniprot/P00722 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/37592181 |
| http://purl.uniprot.org/uniprot/P0A6F9 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/37592181 |