| http://purl.uniprot.org/citations/37620777 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/37620777 | http://www.w3.org/2000/01/rdf-schema#comment | "BackgroundAdipokines, including adiponectin, are implicated in nociceptive pain; however, the underlying cellular and molecular mechanisms remain unknown.MethodsUsing electrophysiological recording, immunostaining, molecular biological approaches and animal behaviour tests, we elucidated a pivotal role of adiponectin in regulating membrane excitability and pain sensitivity by manipulating Cav3.2 channels in trigeminal ganglion (TG) neurons.ResultsAdiponectin enhanced T-type Ca2+ channel currents (IT) in TG neurons through the activation of adiponectin receptor 1 (adipoR1) but independently of heterotrimeric G protein-mediated signaling. Coimmunoprecipitation revealed a physical association between AdipoR1 and casein kinase II alpha-subunits (CK2α) in the TG, and inhibiting CK2 activity by chemical inhibitor or siRNA targeting CK2α prevented the adiponectin-induced IT response. Adiponectin significantly activated protein kinase C (PKC), and this effect was abrogated by CK2α knockdown. Adiponectin increased the membrane abundance of PKC beta1 (PKCβ1). Blocking PKCβ1 pharmacologically or genetically abrogated the adiponectin-induced IT increase. In heterologous expression systems, activation of adipoR1 induced a selective enhancement of Cav3.2 channel currents, dependent on PKCβ1 signaling. Functionally, adiponectin increased TG neuronal excitability and induced mechanical pain hypersensitivity, both attenuated by T-type channel blockade. In a trigeminal neuralgia model induced by chronic constriction injury of infraorbital nerve, blockade of adipoR1 signaling suppressed mechanical allodynia, which was prevented by silencing Cav3.2.ConclusionOur study elucidates a novel signaling cascade wherein adiponectin stimulates TG Cav3.2 channels via adipoR1 coupled to a novel CK2α-dependent PKCβ1. This process induces neuronal hyperexcitability and pain hypersensitivity. Insight into adipoR-Cav3.2 signaling in sensory neurons provides attractive targets for pain treatment."xsd:string |
| http://purl.uniprot.org/citations/37620777 | http://purl.org/dc/terms/identifier | "doi:10.1186/s10194-023-01658-2"xsd:string |
| http://purl.uniprot.org/citations/37620777 | http://purl.uniprot.org/core/author | "Sun Y."xsd:string |
| http://purl.uniprot.org/citations/37620777 | http://purl.uniprot.org/core/author | "Wei Y."xsd:string |
| http://purl.uniprot.org/citations/37620777 | http://purl.uniprot.org/core/author | "Tao J."xsd:string |
| http://purl.uniprot.org/citations/37620777 | http://purl.uniprot.org/core/author | "Tao Y."xsd:string |
| http://purl.uniprot.org/citations/37620777 | http://purl.uniprot.org/core/author | "Zhang Y."xsd:string |
| http://purl.uniprot.org/citations/37620777 | http://purl.uniprot.org/core/author | "Zheng T."xsd:string |
| http://purl.uniprot.org/citations/37620777 | http://purl.uniprot.org/core/author | "Jiang D."xsd:string |
| http://purl.uniprot.org/citations/37620777 | http://purl.uniprot.org/core/date | "2023"xsd:gYear |
| http://purl.uniprot.org/citations/37620777 | http://purl.uniprot.org/core/name | "J Headache Pain"xsd:string |
| http://purl.uniprot.org/citations/37620777 | http://purl.uniprot.org/core/pages | "117"xsd:string |
| http://purl.uniprot.org/citations/37620777 | http://purl.uniprot.org/core/title | "Adiponectin receptor 1-mediated stimulation of Cav3.2 channels in trigeminal ganglion neurons induces nociceptive behaviors in mice."xsd:string |
| http://purl.uniprot.org/citations/37620777 | http://purl.uniprot.org/core/volume | "24"xsd:string |
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