| http://purl.uniprot.org/citations/37644512 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/37644512 | http://www.w3.org/2000/01/rdf-schema#comment | "G4C2 hexanucleotide repeat expansions in a non-coding region of the C9orf72 gene are the most common cause of familial amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). G4C2 insertion length is variable, and patients can carry up to several thousand repeats. Dipeptide repeat proteins (DPRs) translated from G4C2 transcripts are thought to be a main driver of toxicity. Experiments in model organisms with relatively short DPRs have shown that arginine-rich DPRs are most toxic, while polyGlycine-Alanine (GA) DPRs cause only mild toxicity. However, GA is the most abundant DPR in patient brains, and experimental work in animals has generally relied on the use of low numbers of repeats, with DPRs often tagged for in vivo tracking. Whether repeat length or tagging affect the toxicity of GA has not been systematically assessed. Therefore, we generated Drosophila fly lines expressing GA100, GA200 or GA400 specifically in adult neurons. Consistent with previous studies, expression of GA100 and GA200 caused only mild toxicity. In contrast, neuronal expression of GA400 drastically reduced climbing ability and survival of flies, indicating that long GA DPRs can be highly toxic in vivo. This toxicity could be abolished by tagging GA400. Proteomics analysis of fly brains showed a repeat-length-dependent modulation of the brain proteome, with GA400 causing earlier and stronger changes than shorter GA proteins. PolyGA expression up-regulated proteins involved in ER to Golgi trafficking, and down-regulated proteins involved in insulin signalling. Experimental down-regulation of Tango1, a highly conserved regulator of ER-to Golgi transport, partially rescued GA400 toxicity, suggesting that misregulation of this process contributes to polyGA toxicity. Experimentally increasing insulin signaling also rescued GA toxicity. In summary, our data show that long polyGA proteins can be highly toxic in vivo, and that they may therefore contribute to ALS/FTD pathogenesis in patients."xsd:string |
| http://purl.uniprot.org/citations/37644512 | http://purl.org/dc/terms/identifier | "doi:10.1186/s40478-023-01634-6"xsd:string |
| http://purl.uniprot.org/citations/37644512 | http://purl.uniprot.org/core/author | "Zhang P."xsd:string |
| http://purl.uniprot.org/citations/37644512 | http://purl.uniprot.org/core/author | "Pahl A."xsd:string |
| http://purl.uniprot.org/citations/37644512 | http://purl.uniprot.org/core/author | "Partridge L."xsd:string |
| http://purl.uniprot.org/citations/37644512 | http://purl.uniprot.org/core/author | "Isaacs A.M."xsd:string |
| http://purl.uniprot.org/citations/37644512 | http://purl.uniprot.org/core/author | "Gronke S."xsd:string |
| http://purl.uniprot.org/citations/37644512 | http://purl.uniprot.org/core/author | "Moron-Oset J."xsd:string |
| http://purl.uniprot.org/citations/37644512 | http://purl.uniprot.org/core/author | "Jahn A.J."xsd:string |
| http://purl.uniprot.org/citations/37644512 | http://purl.uniprot.org/core/author | "Super T."xsd:string |
| http://purl.uniprot.org/citations/37644512 | http://purl.uniprot.org/core/author | "Fischer L.K.S."xsd:string |
| http://purl.uniprot.org/citations/37644512 | http://purl.uniprot.org/core/author | "Jaure N."xsd:string |
| http://purl.uniprot.org/citations/37644512 | http://purl.uniprot.org/core/date | "2023"xsd:gYear |
| http://purl.uniprot.org/citations/37644512 | http://purl.uniprot.org/core/name | "Acta Neuropathol Commun"xsd:string |
| http://purl.uniprot.org/citations/37644512 | http://purl.uniprot.org/core/pages | "140"xsd:string |
| http://purl.uniprot.org/citations/37644512 | http://purl.uniprot.org/core/title | "Repeat length of C9orf72-associated glycine-alanine polypeptides affects their toxicity."xsd:string |
| http://purl.uniprot.org/citations/37644512 | http://purl.uniprot.org/core/volume | "11"xsd:string |
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