| http://purl.uniprot.org/citations/37684232 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/37684232 | http://www.w3.org/2000/01/rdf-schema#comment | "Many eukaryotic receptors and enzymes rely on glycosylphosphatidylinositol (GPI) anchors for membrane localization and function. The transmembrane complex GPI-T recognizes diverse proproteins at a signal peptide region that lacks consensus sequence and replaces it with GPI via a transamidation reaction. How GPI-T maintains broad specificity while preventing unintentional cleavage is unclear. Here, substrates- and products-bound human GPI-T structures identify subsite features that enable broad proprotein specificity, inform catalytic mechanism, and reveal a multilevel safeguard mechanism against its promiscuity. In the absence of proproteins, the catalytic site is invaded by a locally stabilized loop. Activation requires energetically unfavorable rearrangements that transform the autoinhibitory loop into crucial catalytic cleft elements. Enzyme-proprotein binding in the transmembrane and luminal domains respectively powers the conformational rearrangement and induces a competent cleft. GPI-T thus integrates various weak specificity regions to form strong selectivity and prevent accidental activation. These findings provide important mechanistic insights into GPI-anchored protein biogenesis."xsd:string |
| http://purl.uniprot.org/citations/37684232 | http://purl.org/dc/terms/identifier | "doi:10.1038/s41467-023-41281-y"xsd:string |
| http://purl.uniprot.org/citations/37684232 | http://purl.uniprot.org/core/author | "Chao Y."xsd:string |
| http://purl.uniprot.org/citations/37684232 | http://purl.uniprot.org/core/author | "Li T."xsd:string |
| http://purl.uniprot.org/citations/37684232 | http://purl.uniprot.org/core/author | "Li D."xsd:string |
| http://purl.uniprot.org/citations/37684232 | http://purl.uniprot.org/core/author | "Hong J."xsd:string |
| http://purl.uniprot.org/citations/37684232 | http://purl.uniprot.org/core/author | "Qu Q."xsd:string |
| http://purl.uniprot.org/citations/37684232 | http://purl.uniprot.org/core/author | "Zhang Y."xsd:string |
| http://purl.uniprot.org/citations/37684232 | http://purl.uniprot.org/core/author | "Zhou Z."xsd:string |
| http://purl.uniprot.org/citations/37684232 | http://purl.uniprot.org/core/author | "Xu Y."xsd:string |
| http://purl.uniprot.org/citations/37684232 | http://purl.uniprot.org/core/author | "Zhu Z."xsd:string |
| http://purl.uniprot.org/citations/37684232 | http://purl.uniprot.org/core/date | "2023"xsd:gYear |
| http://purl.uniprot.org/citations/37684232 | http://purl.uniprot.org/core/name | "Nat Commun"xsd:string |
| http://purl.uniprot.org/citations/37684232 | http://purl.uniprot.org/core/pages | "5520"xsd:string |
| http://purl.uniprot.org/citations/37684232 | http://purl.uniprot.org/core/title | "Structures of liganded glycosylphosphatidylinositol transamidase illuminate GPI-AP biogenesis."xsd:string |
| http://purl.uniprot.org/citations/37684232 | http://purl.uniprot.org/core/volume | "14"xsd:string |
| http://purl.uniprot.org/citations/37684232 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/37684232 |
| http://purl.uniprot.org/citations/37684232 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/37684232 |
| http://purl.uniprot.org/uniprot/#_O43292-mappedCitation-37684232 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/37684232 |
| http://purl.uniprot.org/uniprot/#_Q9BZM5-mappedCitation-37684232 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/37684232 |
| http://purl.uniprot.org/uniprot/#_Q9H490-mappedCitation-37684232 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/37684232 |
| http://purl.uniprot.org/uniprot/#_Q96S52-mappedCitation-37684232 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/37684232 |
| http://purl.uniprot.org/uniprot/#_Q92643-mappedCitation-37684232 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/37684232 |
| http://purl.uniprot.org/uniprot/#_Q969N2-mappedCitation-37684232 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/37684232 |