| http://purl.uniprot.org/citations/37684687 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/37684687 | http://www.w3.org/2000/01/rdf-schema#comment | "BackgroundE-proteins encoded by Tcf3, Tcf4, and Tcf12 are class I basic helix-loop-helix (bHLH) transcription factors (TFs) that are thought to be widely expressed during development. However, their function in the developing brain, specifically in the telencephalon remains an active area of research. Our study examines for the first time if combined loss of two E-proteins (Tcf3 and Tcf12) influence distinct cell fates and oligodendrocyte development in the mouse telencephalon.MethodsWe generated Tcf3/12 double conditional knockouts (dcKOs) using Olig2Cre/+ or Olig1Cre/+ to overcome compensatory mechanisms between E-proteins and to understand the specific requirement for Tcf3 and Tcf12 in the ventral telencephalon and during oligodendrogenesis. We utilized a combination of in situ hybridization, immunohistochemistry, and immunofluorescence to address development of the telencephalon and oligodendrogenesis at embryonic and postnatal stages in Tcf3/12 dcKOs.ResultsWe show that the E-proteins Tcf3 and Tcf12 are expressed in progenitors of the embryonic telencephalon and throughout the oligodendrocyte lineage in the postnatal brain. Tcf3/12 dcKOs showed transient defects in progenitor cells with an enlarged medial ganglionic eminence (MGE) region which correlated with reduced generation of embryonic oligodendrocyte progenitor cells (OPCs) and increased expression of MGE interneuron genes. Postnatal Tcf3/12 dcKOs showed a recovery of OPCs but displayed a sustained reduction in mature oligodendrocytes (OLs). Interestingly, Tcf4 remained expressed in the dcKOs suggesting that it cannot compensate for the loss of Tcf3 and Tcf12. Generation of Tcf3/12 dcKOs with Olig1Cre/+ avoided the MGE morphology defect caused by Olig2Cre/+ but dcKOs still exhibited reduced embryonic OPCs and subsequent reduction in postnatal OLs.ConclusionOur data reveal that Tcf3 and Tcf12 play a role in controlling OPC versus cortical interneuron cell fate decisions in MGE progenitors in addition to playing roles in the generation of embryonic OPCs and differentiation of postnatal OLs in the oligodendrocyte lineage."xsd:string |
| http://purl.uniprot.org/citations/37684687 | http://purl.org/dc/terms/identifier | "doi:10.1186/s13064-023-00173-z"xsd:string |
| http://purl.uniprot.org/citations/37684687 | http://purl.uniprot.org/core/author | "Waclaw R.R."xsd:string |
| http://purl.uniprot.org/citations/37684687 | http://purl.uniprot.org/core/author | "Ehrman L.A."xsd:string |
| http://purl.uniprot.org/citations/37684687 | http://purl.uniprot.org/core/author | "Lu Q.R."xsd:string |
| http://purl.uniprot.org/citations/37684687 | http://purl.uniprot.org/core/author | "Nardini D."xsd:string |
| http://purl.uniprot.org/citations/37684687 | http://purl.uniprot.org/core/author | "Talley M.J."xsd:string |
| http://purl.uniprot.org/citations/37684687 | http://purl.uniprot.org/core/date | "2023"xsd:gYear |
| http://purl.uniprot.org/citations/37684687 | http://purl.uniprot.org/core/name | "Neural Dev"xsd:string |
| http://purl.uniprot.org/citations/37684687 | http://purl.uniprot.org/core/pages | "5"xsd:string |
| http://purl.uniprot.org/citations/37684687 | http://purl.uniprot.org/core/title | "Distinct requirements for Tcf3 and Tcf12 during oligodendrocyte development in the mouse telencephalon."xsd:string |
| http://purl.uniprot.org/citations/37684687 | http://purl.uniprot.org/core/volume | "18"xsd:string |
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