| http://purl.uniprot.org/citations/3771528 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/3771528 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/3771528 | http://www.w3.org/2000/01/rdf-schema#comment | "The nucleotide sequence of the malate dehydrogenase (mdh) gene from a thermophilic bacterium, Thermus flavus, was determined. The amino acid sequence of the Thermus malate dehydrogenase resembled that of the porcine heart cytoplasmic enzyme to a certain extent, and Asp-159 and His-187 were identified as possible essential residues for the catalytic function. The mutated mdh gene was also cloned from a spontaneous mutant of T. flavus containing a higher activity of the enzyme. Its mutation point was determined to be a single nucleotide exchange from C to T which caused Thr-190 to be substituted by isoleucine. The mutated enzyme showed resistance to substrate inhibition, an increase in both kcat and Km, and a shift toward a more acid optimum pH for the enzyme reaction."xsd:string |
| http://purl.uniprot.org/citations/3771528 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0021-9258(18)67000-0"xsd:string |
| http://purl.uniprot.org/citations/3771528 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0021-9258(18)67000-0"xsd:string |
| http://purl.uniprot.org/citations/3771528 | http://purl.uniprot.org/core/author | "Nishiyama M."xsd:string |
| http://purl.uniprot.org/citations/3771528 | http://purl.uniprot.org/core/author | "Nishiyama M."xsd:string |
| http://purl.uniprot.org/citations/3771528 | http://purl.uniprot.org/core/author | "Yamamoto K."xsd:string |
| http://purl.uniprot.org/citations/3771528 | http://purl.uniprot.org/core/author | "Yamamoto K."xsd:string |
| http://purl.uniprot.org/citations/3771528 | http://purl.uniprot.org/core/author | "Beppu T."xsd:string |
| http://purl.uniprot.org/citations/3771528 | http://purl.uniprot.org/core/author | "Beppu T."xsd:string |
| http://purl.uniprot.org/citations/3771528 | http://purl.uniprot.org/core/author | "Uozumi T."xsd:string |
| http://purl.uniprot.org/citations/3771528 | http://purl.uniprot.org/core/author | "Uozumi T."xsd:string |
| http://purl.uniprot.org/citations/3771528 | http://purl.uniprot.org/core/author | "Iijima S."xsd:string |
| http://purl.uniprot.org/citations/3771528 | http://purl.uniprot.org/core/author | "Iijima S."xsd:string |
| http://purl.uniprot.org/citations/3771528 | http://purl.uniprot.org/core/author | "Matsubara N."xsd:string |
| http://purl.uniprot.org/citations/3771528 | http://purl.uniprot.org/core/author | "Matsubara N."xsd:string |
| http://purl.uniprot.org/citations/3771528 | http://purl.uniprot.org/core/date | "1986"xsd:gYear |
| http://purl.uniprot.org/citations/3771528 | http://purl.uniprot.org/core/date | "1986"xsd:gYear |
| http://purl.uniprot.org/citations/3771528 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/3771528 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/3771528 | http://purl.uniprot.org/core/pages | "14178-14183"xsd:string |
| http://purl.uniprot.org/citations/3771528 | http://purl.uniprot.org/core/pages | "14178-14183"xsd:string |
| http://purl.uniprot.org/citations/3771528 | http://purl.uniprot.org/core/title | "Nucleotide sequence of the malate dehydrogenase gene of Thermus flavus and its mutation directing an increase in enzyme activity."xsd:string |
| http://purl.uniprot.org/citations/3771528 | http://purl.uniprot.org/core/title | "Nucleotide sequence of the malate dehydrogenase gene of Thermus flavus and its mutation directing an increase in enzyme activity."xsd:string |