| http://purl.uniprot.org/citations/3782074 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/3782074 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/3782074 | http://www.w3.org/2000/01/rdf-schema#comment | "Transcobalamin II (TCII) has been purified from Cohn fraction III of human plasma by batchwise binding to and then elution from carboxymethyl-Sephadex, affinity chromatography using photo-labile aminopropyl cobalamin coupled to activated Sephacryl S-200, and finally chromatography through carboxymethyl cellulose. The yield was approximately 80%. The addition of protease inhibitors in all steps of the purification procedure and extensive washing of the carboxymethyl-Sephadex prior to eluting the TCII minimized degradation of the protein and the final preparation of holo-TCII contained 1 mol of cobalamin/mol of protein. A single polypeptide of 43,000 daltons was obtained by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The NH2-terminal 19 amino acids have been determined for human TCII. 12 of the amino acids are homologous with rabbit TCII and six are homologous with human R-binder, but there is no homology with human intrinsic factor."xsd:string |
| http://purl.uniprot.org/citations/3782074 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0021-9258(18)66733-x"xsd:string |
| http://purl.uniprot.org/citations/3782074 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0021-9258(18)66733-x"xsd:string |
| http://purl.uniprot.org/citations/3782074 | http://purl.uniprot.org/core/author | "Stein S."xsd:string |
| http://purl.uniprot.org/citations/3782074 | http://purl.uniprot.org/core/author | "Stein S."xsd:string |
| http://purl.uniprot.org/citations/3782074 | http://purl.uniprot.org/core/author | "Quadros E.V."xsd:string |
| http://purl.uniprot.org/citations/3782074 | http://purl.uniprot.org/core/author | "Quadros E.V."xsd:string |
| http://purl.uniprot.org/citations/3782074 | http://purl.uniprot.org/core/author | "Rothenberg S.P."xsd:string |
| http://purl.uniprot.org/citations/3782074 | http://purl.uniprot.org/core/author | "Rothenberg S.P."xsd:string |
| http://purl.uniprot.org/citations/3782074 | http://purl.uniprot.org/core/author | "Pan Y.C.E."xsd:string |
| http://purl.uniprot.org/citations/3782074 | http://purl.uniprot.org/core/author | "Pan Y.C.E."xsd:string |
| http://purl.uniprot.org/citations/3782074 | http://purl.uniprot.org/core/date | "1986"xsd:gYear |
| http://purl.uniprot.org/citations/3782074 | http://purl.uniprot.org/core/date | "1986"xsd:gYear |
| http://purl.uniprot.org/citations/3782074 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/3782074 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/3782074 | http://purl.uniprot.org/core/pages | "15455-15460"xsd:string |
| http://purl.uniprot.org/citations/3782074 | http://purl.uniprot.org/core/pages | "15455-15460"xsd:string |
| http://purl.uniprot.org/citations/3782074 | http://purl.uniprot.org/core/title | "Purification and molecular characterization of human transcobalamin II."xsd:string |
| http://purl.uniprot.org/citations/3782074 | http://purl.uniprot.org/core/title | "Purification and molecular characterization of human transcobalamin II."xsd:string |
| http://purl.uniprot.org/citations/3782074 | http://purl.uniprot.org/core/volume | "261"xsd:string |
| http://purl.uniprot.org/citations/3782074 | http://purl.uniprot.org/core/volume | "261"xsd:string |
| http://purl.uniprot.org/citations/3782074 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/3782074 |
| http://purl.uniprot.org/citations/3782074 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/3782074 |