| http://purl.uniprot.org/citations/37823591 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/37823591 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/37823591 | http://www.w3.org/2000/01/rdf-schema#comment | "Chromatin association of the BRCA1-BARD1 heterodimer is critical to promote homologous recombination repair of DNA double-strand breaks (DSBs) in S/G2. How the BRCA1-BARD1 complex interacts with chromatin that contains both damage induced histone H2A ubiquitin and inhibitory H4K20 methylation is not fully understood. We characterised BRCA1-BARD1 binding and enzymatic activity to an array of mono- and di-nucleosome substrates using biochemical, structural and single molecule imaging approaches. We found that the BRCA1-BARD1 complex preferentially interacts and modifies di-nucleosomes over mono-nucleosomes, allowing integration of H2A Lys-15 ubiquitylation signals with other chromatin modifications and features. Using high speed-atomic force microscopy (HS-AFM) to monitor how the BRCA1-BARD1 complex recognises chromatin in real time, we saw a highly dynamic complex that bridges two nucleosomes and associates with the DNA linker region. Bridging is aided by multivalent cross-nucleosome interactions that enhance BRCA1-BARD1 E3 ubiquitin ligase catalytic activity. Multivalent interactions across nucleosomes explain how BRCA1-BARD1 can recognise chromatin that retains partial di-methylation at H4 Lys-20 (H4K20me2), a parental histone mark that blocks BRCA1-BARD1 interaction with nucleosomes, to promote its enzymatic and DNA repair activities."xsd:string |
| http://purl.uniprot.org/citations/37823591 | http://purl.org/dc/terms/identifier | "doi:10.1093/nar/gkad793"xsd:string |
| http://purl.uniprot.org/citations/37823591 | http://purl.org/dc/terms/identifier | "doi:10.1093/nar/gkad793"xsd:string |
| http://purl.uniprot.org/citations/37823591 | http://purl.uniprot.org/core/author | "Burdett H."xsd:string |
| http://purl.uniprot.org/citations/37823591 | http://purl.uniprot.org/core/author | "Burdett H."xsd:string |
| http://purl.uniprot.org/citations/37823591 | http://purl.uniprot.org/core/author | "Kumar D."xsd:string |
| http://purl.uniprot.org/citations/37823591 | http://purl.uniprot.org/core/author | "Kumar D."xsd:string |
| http://purl.uniprot.org/citations/37823591 | http://purl.uniprot.org/core/author | "Wilson M.D."xsd:string |
| http://purl.uniprot.org/citations/37823591 | http://purl.uniprot.org/core/author | "Wilson M.D."xsd:string |
| http://purl.uniprot.org/citations/37823591 | http://purl.uniprot.org/core/author | "Zeqiraj E."xsd:string |
| http://purl.uniprot.org/citations/37823591 | http://purl.uniprot.org/core/author | "Zeqiraj E."xsd:string |
| http://purl.uniprot.org/citations/37823591 | http://purl.uniprot.org/core/author | "Campbell L.J."xsd:string |
| http://purl.uniprot.org/citations/37823591 | http://purl.uniprot.org/core/author | "Campbell L.J."xsd:string |
| http://purl.uniprot.org/citations/37823591 | http://purl.uniprot.org/core/author | "Clifford G."xsd:string |
| http://purl.uniprot.org/citations/37823591 | http://purl.uniprot.org/core/author | "Clifford G."xsd:string |
| http://purl.uniprot.org/citations/37823591 | http://purl.uniprot.org/core/author | "Foglizzo M."xsd:string |
| http://purl.uniprot.org/citations/37823591 | http://purl.uniprot.org/core/author | "Foglizzo M."xsd:string |
| http://purl.uniprot.org/citations/37823591 | http://purl.uniprot.org/core/author | "Heath G.R."xsd:string |
| http://purl.uniprot.org/citations/37823591 | http://purl.uniprot.org/core/author | "Heath G.R."xsd:string |
| http://purl.uniprot.org/citations/37823591 | http://purl.uniprot.org/core/author | "Musgrove L.J."xsd:string |
| http://purl.uniprot.org/citations/37823591 | http://purl.uniprot.org/core/author | "Musgrove L.J."xsd:string |
| http://purl.uniprot.org/citations/37823591 | http://purl.uniprot.org/core/date | "2023"xsd:gYear |
| http://purl.uniprot.org/citations/37823591 | http://purl.uniprot.org/core/date | "2023"xsd:gYear |