http://purl.uniprot.org/citations/37832352 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/37832352 | http://www.w3.org/2000/01/rdf-schema#comment | "BackgroundThe actin regulatory protein fascin (FSCN1) and epithelial mesenchymal transition (EMT) transcription factor (TF) SLUG/SNAI2 have been shown to be expressed in PDAC and its precursor lesions (pancreatic intraepithelial neoplasia (PanIN), graded 1-3) in in vitro and murine in vivo studies. Our aim was to investigate the expression of FSCN1 and EMT-TFs and their association with survival in human PanIN and PDAC.MethodsExpression was investigated in silico using TCGA PanCancer Atlas data (177 PDAC samples with mRNA data) and immunohistochemical staining of a tissue microarray (TMA) (59 PDAC patients).ResultsHigh FSCN1 expression was associated with poorer overall survival (p = 0.02) in the TCGA data. EMT-TF expression was not associated with survival, however FSCN1 expression correlated with that of the EMT-TFs SLUG/SNAI2 (rho = 0.49, p < 0.001) and TWIST1 (rho = 0.52, p < 0.001). TMA IHC showed low expression of SNAI2 and TWIST1 in normal ductal epithelium, while FSCN1 was not expressed. SNAI2 increased slightly in PanIN1-2, then decreased in higher grade lesions. TWIST1 increased in PanIN2-3 and was retained in PDAC. FSCN1 was increasingly expressed from PanIN2 onwards. SNAI2 and TWIST1 expression positively correlated in all grades of PanIN and PDAC (rho = 0.52, p < 0.001). FSCN1 correlated positively with SNAI2 in PanIN1 (rho = 0.56, p < 0.01).ConclusionsIncreased expression of EMT-TFs in low-grade PanIN followed by FSCN1 in PanIN3 and PDAC suggests EMT-TFs may trigger FSCN1 expression and are potential early diagnostic markers. FSCN1 expression correlated with overall survival in PDAC and may have value as a prognostic marker."xsd:string |
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http://purl.uniprot.org/citations/37832352 | http://purl.uniprot.org/core/author | "Machesky L.M."xsd:string |
http://purl.uniprot.org/citations/37832352 | http://purl.uniprot.org/core/author | "Razidlo G.L."xsd:string |
http://purl.uniprot.org/citations/37832352 | http://purl.uniprot.org/core/author | "Bamlet W.R."xsd:string |
http://purl.uniprot.org/citations/37832352 | http://purl.uniprot.org/core/author | "Morris H.T."xsd:string |
http://purl.uniprot.org/citations/37832352 | http://purl.uniprot.org/core/date | "2023"xsd:gYear |
http://purl.uniprot.org/citations/37832352 | http://purl.uniprot.org/core/name | "Pathol Res Pract"xsd:string |
http://purl.uniprot.org/citations/37832352 | http://purl.uniprot.org/core/pages | "154836"xsd:string |
http://purl.uniprot.org/citations/37832352 | http://purl.uniprot.org/core/title | "FSCN1 and epithelial mesenchymal transformation transcription factor expression in human pancreatic intraepithelial neoplasia and ductal adenocarcinoma."xsd:string |
http://purl.uniprot.org/citations/37832352 | http://purl.uniprot.org/core/volume | "251"xsd:string |
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