| http://purl.uniprot.org/citations/37839213 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/37839213 | http://www.w3.org/2000/01/rdf-schema#comment | "Hmi1 is a UvrD-like DNA helicase required for the maintenance of the yeast Saccharomyces cerevisiae mitochondrial DNA (mtDNA). Deletion of the HMI1 ORF leads to the formation of respiration-deficient petite mutants, which either contain a short fragment of mtDNA arranged in tandem repeats or lack mtDNA completely. Here we characterize point mutants of the helicase designed to target the ATPase or ssDNA binding activity and show that these mutations do not separately lead to complete loss of the Hmi1 function. The mutant strains support ATP production via oxidative phosphorylation and enable us to directly analyze the impact of both activities on the stability of wild-type mtDNA in this petite-positive yeast. Our data reveal that Hmi1 mutants affecting ssDNA binding display a stronger defect in the maintenance of mtDNA compared to the mutants of ATP binding/hydrolysis. Hmi1 mutants impaired in ssDNA binding demonstrate sensitivity to UV irradiation and lower levels of Cox2 encoded by the mitochondrial genome. This suggests a complex and multifarious role for Hmi1 in mtDNA maintenance-linked transactions, some of which do not require the ATP-dependent helicase activity."xsd:string |
| http://purl.uniprot.org/citations/37839213 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.dnarep.2023.103582"xsd:string |
| http://purl.uniprot.org/citations/37839213 | http://purl.uniprot.org/core/author | "Sedman J."xsd:string |
| http://purl.uniprot.org/citations/37839213 | http://purl.uniprot.org/core/author | "Sedman T."xsd:string |
| http://purl.uniprot.org/citations/37839213 | http://purl.uniprot.org/core/author | "Joers P."xsd:string |
| http://purl.uniprot.org/citations/37839213 | http://purl.uniprot.org/core/author | "Sillamaa S."xsd:string |
| http://purl.uniprot.org/citations/37839213 | http://purl.uniprot.org/core/author | "Piljukov V.J."xsd:string |
| http://purl.uniprot.org/citations/37839213 | http://purl.uniprot.org/core/author | "Vaask I."xsd:string |
| http://purl.uniprot.org/citations/37839213 | http://purl.uniprot.org/core/date | "2023"xsd:gYear |
| http://purl.uniprot.org/citations/37839213 | http://purl.uniprot.org/core/name | "DNA Repair (Amst)"xsd:string |
| http://purl.uniprot.org/citations/37839213 | http://purl.uniprot.org/core/pages | "103582"xsd:string |
| http://purl.uniprot.org/citations/37839213 | http://purl.uniprot.org/core/title | "UvrD-like helicase Hmi1 Has an ATP independent role in yeast mitochondrial DNA maintenance."xsd:string |
| http://purl.uniprot.org/citations/37839213 | http://purl.uniprot.org/core/volume | "132"xsd:string |
| http://purl.uniprot.org/citations/37839213 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/37839213 |
| http://purl.uniprot.org/citations/37839213 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/37839213 |
| http://purl.uniprot.org/uniprot/#_A0A8H4BUH2-mappedCitation-37839213 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/37839213 |
| http://purl.uniprot.org/uniprot/#_Q12039-mappedCitation-37839213 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/37839213 |
| http://purl.uniprot.org/uniprot/A0A8H4BUH2 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/37839213 |
| http://purl.uniprot.org/uniprot/Q12039 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/37839213 |