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http://purl.uniprot.org/citations/37858140http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/37858140http://www.w3.org/2000/01/rdf-schema#comment"

Background

Circular RNAs are involved in autoimmune disease pathogenesis. Our previous study indicated that circPTPN22 is involved in autoimmune diseases, such as systemic lupus erythematosus (SLE) and rheumatoid arthritis, but the underlying mechanisms remain unclear.

Methods

First, the expression of circPTPN22 was detected by real-time PCR and western blotting. After overexpression or knockdown of circPTPN22, the proliferation of Jurkat cells was detected by the CCK-8 assay, and the apoptosis of Jurkat cells was detected by flow cytometry. In addition, the relationship between circPTPN22-miR-4689-S1PR1 was confirmed by bioinformatic analyses, fluorescence in situ hybridization assays, RNA-binding protein immunoprecipitation, and dual luciferase reporter assays.

Results

We found that circPTPN22 expression was downregulated in the PBMCs of SLE patients compared to those of healthy controls. Overexpression of circPTPN22 increased proliferation and inhibited apoptosis of Jurkat T cells, whereas knockdown of circPTPN22 exerted the opposite effects. CircPTPN22 acts as a miR-4689 sponge, and S1PR1 is a direct target of miR-4689. Importantly, the circPTPN22/miR-4689/S1PR1 axis inhibited the secretion of TNF-α and IL-6 in Jurkat T cells.

Conclusions

CircPTPN22 acts as a miR-4689 sponge to regulate T-cell activation by targeting S1PR1, providing a novel mechanism for the pathogenesis of SLE."xsd:string
http://purl.uniprot.org/citations/37858140http://purl.org/dc/terms/identifier"doi:10.1186/s13075-023-03150-3"xsd:string
http://purl.uniprot.org/citations/37858140http://purl.uniprot.org/core/author"Chen X."xsd:string
http://purl.uniprot.org/citations/37858140http://purl.uniprot.org/core/author"Jia Y."xsd:string
http://purl.uniprot.org/citations/37858140http://purl.uniprot.org/core/author"Li S."xsd:string
http://purl.uniprot.org/citations/37858140http://purl.uniprot.org/core/author"Wu Q."xsd:string
http://purl.uniprot.org/citations/37858140http://purl.uniprot.org/core/author"Xiao J."xsd:string
http://purl.uniprot.org/citations/37858140http://purl.uniprot.org/core/author"Zhang M."xsd:string
http://purl.uniprot.org/citations/37858140http://purl.uniprot.org/core/author"Tang J."xsd:string
http://purl.uniprot.org/citations/37858140http://purl.uniprot.org/core/author"Zhong Z."xsd:string
http://purl.uniprot.org/citations/37858140http://purl.uniprot.org/core/author"Jiang Z."xsd:string
http://purl.uniprot.org/citations/37858140http://purl.uniprot.org/core/author"Zhai Z."xsd:string
http://purl.uniprot.org/citations/37858140http://purl.uniprot.org/core/author"Miao Q."xsd:string
http://purl.uniprot.org/citations/37858140http://purl.uniprot.org/core/author"Ni B."xsd:string
http://purl.uniprot.org/citations/37858140http://purl.uniprot.org/core/date"2023"xsd:gYear
http://purl.uniprot.org/citations/37858140http://purl.uniprot.org/core/name"Arthritis Res Ther"xsd:string
http://purl.uniprot.org/citations/37858140http://purl.uniprot.org/core/pages"206"xsd:string
http://purl.uniprot.org/citations/37858140http://purl.uniprot.org/core/title"CircPTPN22 modulates T-cell activation by sponging miR-4689 to regulate S1PR1 expression in patients with systemic lupus erythematosus."xsd:string
http://purl.uniprot.org/citations/37858140http://purl.uniprot.org/core/volume"25"xsd:string
http://purl.uniprot.org/citations/37858140http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/37858140
http://purl.uniprot.org/citations/37858140http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/37858140
http://purl.uniprot.org/uniprot/#_A0A0B4J1S7-mappedCitation-37858140http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/37858140
http://purl.uniprot.org/uniprot/#_A0A1Q2XD95-mappedCitation-37858140http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/37858140
http://purl.uniprot.org/uniprot/#_A0A1Q2XDC9-mappedCitation-37858140http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/37858140
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