| http://purl.uniprot.org/citations/37866301 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/37866301 | http://www.w3.org/2000/01/rdf-schema#comment | "In this study, we aimed to investigate the effect of the expression of miR-155-5p and its target genes on oocyte maturation. We analyzed the expression of miR-155-5p and its target genes in cumulus cells and oocytes using quantitative real-time reverse-transcription polymerase chain reaction. Using carboxyfluorescein, porcine cumulus cells were transfected with mimics and inhibitors of ssc-miR-155-5p to induce in vitro maturation, and subsequently, cumulus expansion, oocyte maturation, and cleavage rate were measured. We found that miR-155-5p expression in cumulus cells at the metaphase II stage was significantly higher than that at the germinal vesicle (GV) stage, whereas Cx43 expression was significantly lower than that at the GV stage (P < 0.05). Compared with those in the negative control group, the cumulus diffusion area of cumulus oocyte complexes; oocyte maturation rate; cleavage rate; HAS2, PTGS2, CD44, PTX3, and TNFAIP6 expression in cumulus cells; and GDF9, BMP15, CyclinB1, and CDK1 expression in oocytes were significantly increased in the miR-155-5p mimics group (P < 0.05), whereas the mRNA and protein expression of CX43 were significantly decreased (P < 0.05). Compared with that in the negative control group, the protein expression of CyclinB1 and p-CDK1 (Thr14, Tyr15) in the miR-155-5p mimics group was significantly increased (P < 0.05). These results suggest that miR-155-5p regulates maturation promoting factor activity by targeting Cx43, which improves the in vitro maturation and cleavage rate of porcine oocytes."xsd:string |
| http://purl.uniprot.org/citations/37866301 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.theriogenology.2023.10.013"xsd:string |
| http://purl.uniprot.org/citations/37866301 | http://purl.uniprot.org/core/author | "Chen X."xsd:string |
| http://purl.uniprot.org/citations/37866301 | http://purl.uniprot.org/core/author | "Han Y."xsd:string |
| http://purl.uniprot.org/citations/37866301 | http://purl.uniprot.org/core/author | "Han M."xsd:string |
| http://purl.uniprot.org/citations/37866301 | http://purl.uniprot.org/core/author | "Jin Y."xsd:string |
| http://purl.uniprot.org/citations/37866301 | http://purl.uniprot.org/core/author | "Cheng M."xsd:string |
| http://purl.uniprot.org/citations/37866301 | http://purl.uniprot.org/core/author | "Lv Y."xsd:string |
| http://purl.uniprot.org/citations/37866301 | http://purl.uniprot.org/core/author | "Luo X."xsd:string |
| http://purl.uniprot.org/citations/37866301 | http://purl.uniprot.org/core/author | "Wang M."xsd:string |
| http://purl.uniprot.org/citations/37866301 | http://purl.uniprot.org/core/author | "Zhang J."xsd:string |
| http://purl.uniprot.org/citations/37866301 | http://purl.uniprot.org/core/author | "Yu Y."xsd:string |
| http://purl.uniprot.org/citations/37866301 | http://purl.uniprot.org/core/author | "Cao L."xsd:string |
| http://purl.uniprot.org/citations/37866301 | http://purl.uniprot.org/core/date | "2024"xsd:gYear |
| http://purl.uniprot.org/citations/37866301 | http://purl.uniprot.org/core/name | "Theriogenology"xsd:string |
| http://purl.uniprot.org/citations/37866301 | http://purl.uniprot.org/core/pages | "124-133"xsd:string |
| http://purl.uniprot.org/citations/37866301 | http://purl.uniprot.org/core/title | "miR-155-5p improves oocyte maturation in porcine cumulus cells through connexin 43-mediated regulation of MPF activity."xsd:string |
| http://purl.uniprot.org/citations/37866301 | http://purl.uniprot.org/core/volume | "214"xsd:string |
| http://purl.uniprot.org/citations/37866301 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/37866301 |
| http://purl.uniprot.org/citations/37866301 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/37866301 |
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| http://purl.uniprot.org/uniprot/#_X5FBP6-mappedCitation-37866301 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/37866301 |