| http://purl.uniprot.org/citations/37923757 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/37923757 | http://www.w3.org/2000/01/rdf-schema#comment | "The Gram-positive spore-forming bacterium Bacillus anthracis is the causative agent of anthrax, a deadly disease mostly affecting wildlife and livestock, as well as representing a bioterrorism threat. Its cell surface is covered by the mutually exclusive S-layers Sap and EA1, found in early and late growth phases, respectively. Here we report the nanobody-based structural characterization of EA1 and its native lattice contacts. The EA1 assembly domain consists of 6 immunoglobulin-like domains, where three calcium-binding sites structure interdomain contacts that allow monomers to adopt their assembly-competent conformation. Nanobody-induced depolymerization of EA1 S-layers results in surface defects, membrane blebbing and cell lysis under hypotonic conditions, indicating that S-layers provide additional mechanical stability to the cell wall. Taken together, we report a complete model of the EA1 S-layer and present a set of nanobodies that may have therapeutic potential against Bacillus anthracis."xsd:string |
| http://purl.uniprot.org/citations/37923757 | http://purl.org/dc/terms/identifier | "doi:10.1038/s41467-023-42826-x"xsd:string |
| http://purl.uniprot.org/citations/37923757 | http://purl.uniprot.org/core/author | "Steyaert J."xsd:string |
| http://purl.uniprot.org/citations/37923757 | http://purl.uniprot.org/core/author | "Pardon E."xsd:string |
| http://purl.uniprot.org/citations/37923757 | http://purl.uniprot.org/core/author | "Remaut H."xsd:string |
| http://purl.uniprot.org/citations/37923757 | http://purl.uniprot.org/core/author | "Sogues A."xsd:string |
| http://purl.uniprot.org/citations/37923757 | http://purl.uniprot.org/core/author | "Fioravanti A."xsd:string |
| http://purl.uniprot.org/citations/37923757 | http://purl.uniprot.org/core/author | "Jonckheere W."xsd:string |
| http://purl.uniprot.org/citations/37923757 | http://purl.uniprot.org/core/date | "2023"xsd:gYear |
| http://purl.uniprot.org/citations/37923757 | http://purl.uniprot.org/core/name | "Nat Commun"xsd:string |
| http://purl.uniprot.org/citations/37923757 | http://purl.uniprot.org/core/pages | "7051"xsd:string |
| http://purl.uniprot.org/citations/37923757 | http://purl.uniprot.org/core/title | "Structure and function of the EA1 surface layer of Bacillus anthracis."xsd:string |
| http://purl.uniprot.org/citations/37923757 | http://purl.uniprot.org/core/volume | "14"xsd:string |
| http://purl.uniprot.org/citations/37923757 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/37923757 |
| http://purl.uniprot.org/citations/37923757 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/37923757 |
| http://purl.uniprot.org/uniprot/#_P94217-mappedCitation-37923757 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/37923757 |
| http://purl.uniprot.org/uniprot/P94217 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/37923757 |